User:Cassandra M Barrett/Notebook/Haynes Lab/2015/08/21

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T2A-EGFP Phusion PCR

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Purpose: To amplify T2A-EGFP fragment from pX330g plasmid for insertion into new vector

Materials: Primers P231/P232 pX330g Phusion Reagents

Methods:

Tempate was diluted 1:1000

Set up reaction as follows

Water 32.5uL HFmm 10uL dNTPs 1uL Forward Primer 2.5uL Reverse Primer 2.5uL Template 1uL Phusion Enzyme 0.5uL

Run on Phusion PCR program at 60C

Results: Gel revealed failed PCR, reattempt



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