User:Carly M. Montanero/Notebook/CHEM-571/2014/03/25

Biomaterials Design Lab: Spring 2014

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Objective Add three concentrations (30, 60, 90 mM) of variables to the 30:1 lysozyme-AuNP solutions at three temperatures (4°C, 22°C, 36°C). Synthesize an additional tube of 30:1 lysozyme-AuNP to use as a standard for future testing. Procedure Preparing Samples for Addition of Variables Pipette 5 mL aliquots into a Falcon tube from the 15 mL samples. Dilution of Variables The following table shows how we diluted the stock concentrations of various variables. The first graph shows the dilution to 30 mM, the second graph shows 60 mM, and the third graph shows 90 mM. The concentrations of 2,2 bipyridine used are 0.0002316 M, 0.0004544 M, and 0.0006772 M from a stock concentration of 0.0099 M. Adding Variables to 30:1 Lysozyme-AuNP Solutions Add 2.5 mL of each variable to each tube. Place 21 tubes in the oven at 36°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine. Place 21 tubes in the freezer at 4°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine. Place 21 tubes on the bench at 22°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine. Synthesizing 30:1 Lysozyme-AuNP The following figure describes how we made one tube of 30:1 lysozyme-AuNPs. The sample was heated at 80°C for 4 hours.