User:Carly M. Montanero/Notebook/CHEM-571/2013/11/13

Biomaterials Design Lab: Fall 2013

Main project page Previous entry      Next entry

Objective To run atomic absorption (AA) on our lysozyme and myoglobin and hemoglobin samples previously synthesized. Procedure Creating the Gold Stock Solutions Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. Atomic Absorption Samples Solutions with the following Au:protein ratio were run on the AA: Gold to Hemoglobin Ratio 30 50 70 90 110 130 150 170 190 210 Gold to Lysozyme Ratio 5 10 15 20 25 30 35 40 45 50 Gold to Lysozyme Ratio 30 50 70 90 110 130 150 170 190 210 Gold to Myoglobin Ratio 10 15 25 30 35 40 45 50 Figures