User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29

Biomaterials Design Lab: Fall 2013

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Objective Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs. Procedure Creating the Gold Stock Solution Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water. The final concentration of gold stock was 2.57 mM. Creating the Lysozyme Stock Solution Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water. The final concentration of lysozyme stock was 15 μM. Creating the Myoglobin Stock Solution Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water. The final concentration of myoglobin stock was 15 μM. Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio were synthesized: Gold to Lysozyme Ratio 30 50 70 90 110 130 150 170 190 210 Gold to Myoglobin Ratio 10 15 25 30 35 40 45 50 As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. The samples were heated at 80°C for 4 hours. Figures Myoglobin UV-Vis Lysozyme UV-Vis Notes The final gold concentration in the solutions was 0.25 mM.