User:Burak Yilmaz/Notebook/YR10 protein interaction/2009/09/22

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plasmid concentrator

  • using zymo kit

Protocol

  • 1. Add two volumes of DNA Binding Buffer to each volume of DNA sample. (e.g.,

300 μl DNA Binding Buffer to 150 μl DNA sample). Transfer sample mixtures to the wells of a Zymo-Spin I-96 Plate™ mounted on a Collection Plate. Alternatively, add two volumes of DNA Binding Buffer to each volume of DNA sample directly in the wells of the Zymo-Spin I-96 Plate™ mounted on a Collection Plate. Ensure proper mixing by pipetting up and down a few times.

  • 2. Centrifuge at 2,500-8,000 rpm (minimum 1000 x g) for 5 minutes until sample

mixtures have been completely filtered. Discard the flow-through.

  • 3. Add 300 μl Wash Buffer to each well of the Zymo-Spin I-96 Plate™.

Centrifuge at 2,500-8,000 rpm for 5 minutes. Repeat wash step. (Alternatively, one wash can be performed using 600 μl Wash Buffer).

  • 4. Add 10-15 μl water directly to the column matrix in each well. Transfer the

Zymo-Spin I-96 Plate™ onto an Elution Plate and centrifuge at 2,500-8,000 rpm for 5 minutes to elute the DNA. Ultra-pure DNA in water is now ready for use.



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