User:Brian P. Josey/Notebook/2009/12/01

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Ligation and Tethering

We are doing the ligation reaction and tethering today. We started off with the ligation, where we mixed together 25μL of the pBR322, 4.25μL anchor, 2μL of the adapter, 5μL of the buffer, 1μL of DNA ligase and 6.25μL of the DI water. We ran this in at 16°C and added 2.0 μL of the adapter at twenty minute increments, giving an final eventual volume of 8μL Here is Anthony's table showing the values:

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