| 050607Chemo12hr.PNG: | | 06.07.2005 Chemostat Run |
| 07.06.13.jpg: | | Gel image |
| 070909-pcr assembly.jpg: | | PCR fusion test gel |
| 070909-pcr assemblyB.jpg: | | |
| 2005.06.07.ChemostatRun.JPG: | | 06.07.2005 Chemostat Run |
| 2005.4.5-2.jpg: | | Closeup of pump with cells |
| 702px-I13600 - UV 254nm.jpg: | | |
| 90hrRunf.PNG: | | 90hr chemostat run |
| Anthropology.PNG: | | anthropolgy home page pic |
| BEBoard.JPG: | | BE Board Logo |
| BEING-leaf-logo.jpg: | | Logo for BEInG front page |
| BEInG lab.jpg: | | Setup for BEInG Hydrogen Bioreactor 9/26/2005 |
| BarberoECDimage.JPG: | | Image from Robbie Barbero, Grad student at MIT, from virus-based electrochromic devices that can change from clear to opaque when a charge is applied. |
| BeingalgaeSetup.jpg: | | BEInG's Setup for growing algae to make H2. |
| Bioreactor-schematic.jpg: | | Schematic of a hydrogen photobioreactore
(BEInG) |
| CFP-YFP plate reader comparison.PNG: | | Comparison of CFP and YFP expressing cells in the plate reader. |
| CHR31044v2.gif: | | Chroma spectra 31044v2 |
| CHR41001.PNG: | | Chroma spectra 41001 |
| CHR41028.PNG: | | 41028 Chroma Spectra |
| CHR41043.gif: | | Chroma spectra 41043 |
| Changing Sensor Zero Values.pdf: | | document for changing sensor zero values on solent incubator. |
| ChemoIP1 orig bright.jpg: | | Chemostat IP orignal brightened |
| ChemoIP1 orig.jpg: | | chemostat image process original |
| ChemoIP1 process.jpg: | | chemostat image process processed |
| Csbi.PNG: | | MIT CSBI logo |
| DNA only horizontal.png: | | no circle around emblem horizontal |
| DNANanodropExample1.PNG: | | Example of wavelength vs. absorbance curve for a miniprep sample on the nanodrop. |
| DNAonly vertical.png: | | shirt with no circle around logo |
| DelLacYInduction.PNG: | | Induction of lac-regulated promoter in lacY- and lacY+ strains, from Jensen et al. Eur.J.Biochem, 211, 181-191 (1993) |
| ESP-hairpinTest.PNG: | | Experiment to test hairpin effectiveness in the empty screening plasmid. |
| ESP-promoterTest.PNG: | | Results of experiment by Jason Kelly to look at possible promoter in the empty screening plasmid |
| Ecoli.jpg: | | A plush microbe from Giant Microbes.com |
| Endy NikonTE2000.jpg: | | Picture of Endy Lab Microscope |
| EndyCirculatorManual.pdf: | | Manual for the Polyscienec Circulator. |
| Example.jpg: | | Image:IGEM.Kyoto leaflet.pdf |
| Fig1-becskei2000.PNG: | | figure 1 from becskei and serrano, nature 2000. |
| FreeGenes.JPG: | | |
| GFP RBS relative dgfp 080204c.jpg: | | RBS tester |
| GFPstandardProcess.PNG: | | process for GFP measurement standardization work |
| Ginkgologo.PNG: | | |
| GrowthIssues.JPG: | | Graph of cells dying out insortostat in long runs. |
| Harris-mage.PNG: | | harris presents MAGE |
| Headbg.jpg: | | banner |
| I13534vsI13457.JPG: | | another test |
| ICampus-SpringReview-FINAL.doc: | | Word version of the OWW iCampus spring review for backup. |
| IGEM-cropped-edited.PNG: | | picture of MIT home page w 2006 iGEM team on it |
| IGEM06-Mint.JPG: | | iGEM results for mint |
| Icampus.PNG: | | MIT iCampus Logo |
| JK BE Retreat 2006.pdf: | | BE Retreat 2006 poster |
| JK ICSB 2005.pdf: | | Jason Kelly ICSB 2005 Poster |
| JK.BERetreat.Poster.2007.pdf: | | Poster presented by Jason Kelly at MIT BE retreat 3/2007 |
| JK.SB3.0.Poster.2007.pdf: | | Jason Kelly SB3.0 Poster |
| JK.SyntheticBiology2.0.Poster.pdf: | | Jason Kelly's Synthetic Biology 2.0 Poster |
| JKM Glycerols.xls: | | JKM glycerols from endy lab |
| Jkpic.JPG: | | be directory photo |
| July8 2006 gel.jpg: | | |
| KC Glycerols.xls: | | KC glycerols |
| KlettvsCellDensity.png: | | Calibration Curve for Klett vs. Cell Density |
| LacI-MullerHill94.JPG: | | Figure 1 from Muller-Hill et al. EMBO J. 1994 July 15; 13(14): 3348–3355. |
| Luciferyeast.JPG: | | luciferyeast |
| MFruit Curves.PNG: | | mFRuit curves with 568nm laster line approximately positioned on the graph. Image comes from Shaner et al, Nature Biotech, Nov 2004. |
| MFruit Curves.bmp: | | this is a test |
| MOFLODarkNoise.jpg: | | Example of "dark noise" on non-flourescent cells on the MOFLO as a result of high PMT settings. (A) FL1=max / FL7=max (B) FL1 = 525 / FL7=max (C) FL1=525 / FL7 = 650. |
| MOFLObeads.PNG: | | Example of what Spherotech RCP-60-5 Rainbow Calibration Particles (6.0-6.4um) should look like on the MOFLO under good alignment. |
| MRFP1.JPG: | | mRFP1 spectra |
| MeasKit inputs.JPG: | | inputs to the measurement kit run on a gel to verify length and digestion |
| Measkit promoter FACS.PNG: | | |
| MntInverter.png: | | Mnt-based inverter in SP1.0 |
| MofloBEADS-070520.png: | | Example of what Spherotech RCP-60-5 Rainbow Calibration Particles (6.0-6.4um) should look like on the MOFLO under good alignment. 05/20/07 |
| Moss1.jpg: | | |
| Moss2.jpg: | | |
| Moss3.jpg: | | |
| Moss5.jpg: | | |
| Nature Presentation - Feb5-07.ppt: | | Presentation to NPG NY office on Feb 5, 2007 by Sri, Jason, and Drew. |
| Nicholson1997Fig1.PNG: | | Figure 1 from Zhang and Nicholson, PNAS 1997.
(A) Substrate alignment analysis. Ten substrates were aligned whose cleavage sites were accurately determined, either by direct RNA sequence analysis or by primer extension of RNA cleaved in vitro by purified RNase III. (B) The “disfavored” bp, displayed in a dsRNA structure. The proximal box (PB) and distal box (DB) are included within an 11-bp helix. S = C or G, with S′ complementary to S. N, N′ indicate complementary nucleotides; while n, n′ indicate less strict complementarity. Arrowheads indicate the (blocked) cleavage sites. (C) Absence of conservation (degeneracy) of RNase III substrate sequence. H = A, G, U, with D′ (A, C, U) complementary to H; B = C, G, U, with V′ (G, C, U) complementary to B; W, W′ = A, U. (D) Secondary structure of the T7 R1.1 RNase III substrate, showing the proximal and distal boxes and the single cleavage site (arrowhead). |
| OD600vsCellDensity.JPG: | | OD600 vs. CFU density |
| OWW round.jpg: | | Higher resolution copy of the sticker. |
| OWW tshirt.jpg: | | OWW tshirt option |
| OWW-mithomepage.PNG: | | OpenWetWare on the MIT homepage |
| OpenWetWare iCampus Spring Review.pdf: | | OpenWetWare Spring Review for iCampus Grant |
| Oww onOLPC.jpg: | | OWW on the OLPC XO computer |
| PACCAR16.JPG: | | pACCAR16 structure |
| Patent squad memo-Breetz-1.doc: | | Memo by Hanna Breetz evaluating the viability of the patent goon squad |
| Promoter table.JPG: | | from Hawley & McClure, NAR Vol 11 (8) 1983
Table of nucleotide frequencies in the consensus e.coli sigma70 promoter |
| Promoter tester demo.jpg: | | Variety of constitutive promoters inserted into the promoter measurement kit. |
| PromoterChar Report Revised.doc: | | doc on promoter char by Caitlin Conboy |
| PromotersInReg.JPG: | | |
| Proposed promoter standard.JPG: | | proposed promoter standard with fixed +1 site |
| Proposed promoter standard2.JPG: | | another option for BB promoter standard. |
| Psb3k3 Pcr prep.JPG: | | preparatory PCR of psb3K3 on gel |
| Q04400InverterInSP1.0.PNG: | | Q04400 in SP1.0 |
| Q04400LibInSP1.0.PNG: | | Q04400 library in Sp1.0 |
| Q04401InSp1.0.PNG: | | Q04401 (mutant isolate from Q04400 library) in SP1.0 |
| Q04740sp.PNG: | | q04740 characterization with SP1.0 |
| RBS tester colony PCR.JPG: | | Colony PCR of 15 colonies following a construction using the RBS Measurement Kit |
| Repressor sites.gif: | | |
| SSchemo.PNG: | | early chemostat results |
| ScreeningPlasmid1.0.PNG: | | Screening Plasmid 1.0 |
| ScreeningPlasmidDesign.PNG: | | Schematic and actual design for the screening plasmid |
| ScreeningPlasmidSchematic.PNG: | | Schematic of Screening Plasmid |
| Seq1.jpg: | | seq of library |
| SortoStatDesign.PNG: | | Sortostat design image |
| Sortostat f2620.PNG: | | |
| Sortostat v2.0 - whiteBG.JPG: | | AutoCAD image of the Sortostat v2.0 design on white background with layers colored (blue = fluid, red = push UP, green = push DOWN) |
| Sortostat v2.0.PNG: | | Autocad image of Sortostat v2.0 |
| Sortostat-valves-partialClosed.PNG: | | Example of valves not sealing properly due to fabrication problems. |
| Spec calibration.xls: | | test of excel upload |
| Streak plates.png: | | Agar plate being streaked with bacteria. Drawn by Theresa Knott. thanks wikipedia! |
| SynBERC talk Reisinger.ppt: | | Sarah Reisinger Talk for SynBERC slides from Leonard Katz |
| TermDotPLot.JPG: | | Dot plot of terminators characterizaed by screening plasmid 1.0 |
| TermsCorrected.jpg: | | Terminator characterization with Screening Plasmid 1.0 |
| TestImage.PNG: | | test Image |
| Torch-01-june.gif: | | torch |
| UT HelloWorld.jpg: | | UT austin biofilm from wikipedia |
| Valves-partialClosed.PNG: | | partially closed valves |
| Weiss-sbreview-f1.jpg: | | Figure 1 from Andrianantoandro et al MSB 2006 (permission granted for re-use from NPG and EMBO). |
| Weiss-sbreview-f2.jpg: | | Figure 2 from Andrianantoandro et al MSB 2006 (permission granted for re-use from NPG and EMBO). |
