User:Benjamin Friedel/Notebook/CHEM 471/2016/02/17

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Objectives

  1. Set up 1ml AgNP and AuNP synthesis reactions for SPI, WPI, Casein, Pea Protein and Brown Rice Protein
  2. Set up 20ml Pea Protein AgNP and Brown Rice Protein AgNP reactions
  3. Analyze Reactions from 02/16/16 with UV Vis

Materials

  1. Used same nutritional proteins on 02/02/16
  2. Protein and gold stocks prepared fresh every day
  3. Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
    1. Integration time= 10ms and Average =100.
    2. A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.

Procedure

  1. Further reduce stock protein concentrations (and thus concentrations in reaction mix) to increase metal/protein ratio to spur NP formation as we saw protein aggregate and nanofiber formation in previous reactions
  2. increase concentration from 02/16/16 trial
  3. comparing citrate to no citrate samples, and vary citrate concentration
  1. Prepare 20ml reaction to synthesize large quantities of reactions known to work
    1. Pea Protein and Brown Rice Protein AgNP
      1. Note that spectra of reaction mixes before reacting need to be taken
      2. Also note that this is just one size of nanoparticle that can be made with these reactions, its possible to change variables and change size and functionality of nanoparticle, these were just the first reactions to work.


Detailed Reaction Setup shown Below (4 hours 80˚C, 1ml rxns)

20 mL Reactions ' ' ' ' ' ' ' '
Vial #2.5mM HAuCl4 (µL)5 mMAgNO3 (µL)Protein (µL)10 mM Citrate (µL)Water (µL)Worked?Notes
Casein1?0?
5 mg/mL208001120008000Run UV/Vis
Soy38000400040014800Run UV/Vis
0.25 mg/mL40??0
Whey58000400040014800Run UV/Vis
0.25 mg/mL60??0
Pea7?0?
0.15 mg/mL808004000015200Redo
Rice9?0?
0.15 mg/mL1008004000015200
1mL Reactions
Vial #5.0 mM HAuCl4 (µL)5.0 mMAgNO3 (µL)Protein (µL)10 mM Citrate (µL)Water (µL)Worked?Notes
14002000760
0.25 mg/mL Casein240020020740
Stock: 15mL340020040720
440020060700
50402000760
604020080680
0.25 mg/mL Soy7040200100660
Stock: 10mL8040200150610
9040200200560
100402000760
1104020080680
0.25 mg/mL Whey12040200100660
Stock: 10mL13040200150610
14040200200560
154002000760
0.15 mg/mL Pea1640020020740
Stock: 22 mL1740020040720
5.48g1840020060700
194002000760
0.15 mg/mL Rice2040020020740
Stock: 27mL2140020040720
6.75g2240020060700

Results

20ml Samples

  1. Brown Rice Protein AgNP solution turned gold after reaction, even before reaction started after 10 minutes at room temperature started to form AgNPs and turned slightly yellow
  2. Pea Protein solution remained clear with some white precipitation, possibly not total dilution

UV-Vis data at SRB Lab Notebook Data


Personal tools