User:Benjamin Friedel/Notebook/472 CHEM/2016/01/26

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Objective

Todays objective is to prepare gold and silver nanoparticles using pure casein and soy protein isolate as capping and reducing agents

Materials

Stock Solutions

  1. SPI (ARCON S 066400)
  1. Casein (amresco E666-500G)
    1. note store in refrigerator
  1. Fructose
  1. Choloroauric Acid (REM Chemicals)
    1. note store in desiccator, sensitive to metals
  1. Silver Nitrate (CMI 7985)
    1. note store in desiccator, sensitive to light

Procedure

  • Gold Nanoparticles

Procedure to make the gold nanoparticles was based off of Dr. Hartings' notebook. Instead of lysozyme, Casein (an estimated MW at 22kD) was used. Then the concentration of Casein for 20uM was converted to g/ml, and this concentration was applied to the soy protein isolate (SPI) which does not have a clear MW.

The reaction spreadsheet is shown below. After creating the reaction mixes, samples were heated at 80˚C for 4 hours in the HERATHERM oven in Dr. Harting's Lab in Beeghly (on AU campus). This reaction cycle also consisted of 30 minutes of heating and cooling beforehand. Samples were retrieved the next day (1/27/16), checked for color change and then covered in tin foil completely (to prevent oxidation by light of potentially unreacted silver nitrate).


1/27/2016 ' ' ' ' ' ' ' ' '
1mL total reaction2.5mM HAuCl42.5mMAgNO3.44mg/ml SPI.44mg/ml Casein2.5mM FructoseWaterAu/Ag:ProteinNotes
140µL00560µL0400µL1:15no color change
240µL00440µL0520µL1:11
340µL00360µL0600µL1:09
440µL00280µL0680µL1:07
540µL00200µL0760µL1:05
6040µL0560µL0uL400µL1:15all gold/bright yellow except for #14 which was lighter
7040µL0560µL20uL380µL1:15
8040µL0560µL40uL360µL1:15
9040µL0360uL0uL600µL1:09
10040µL0360µL20uL580µL1:09
11040µL0360µL40uL560µL1:09
12040µL0200uL0uL760µL1:05
13040µL0200µL20uL740µL1:05
14040µL0200µL40uL720µL1:05
1540µL0560µL00400µL1:15Made fibers so need higher concentration of protein
1640µL0440µL00520µL1:11purple fibers at bottom
1740µL0360µL00600µL1:09
1840µL0280µL00680µL1:07
1940µL0200µL00760µL1:05
20040µL560µL00uL400µL1:15No color change
21040µL560uL020uL380µL1:15
22040µL560µL040uL360µL1:15
23040µL360uL00uL600µL1:09
24040µL360µL020uL580µL1:09
25040µL360µL040uL560µL1:09
26040µL200uL00uL760µL1:05
27040µL200µL020uL740µL1:05
28040µL200µL040uL720µL1:05
Controls 2940µL0000960µLNo color change
30040µL000960µL
-oven started 4:35pm 1/27, removed and covered 10:30am 1/28


  • Silver nanoparticles

Results

  1. Silver nanoparticles with casein were created as seen by a color change from clear to yellow, as well as a clear and solitary peak at 420nm in UV Vis.



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