User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/09

PcTF Subcloning in E. coli

Main project page Previous entry      Next entry

08/09/2013 Flourescent Microscopy Check RFP/CFP of co-transfection after 18 hrs. BioBrick Part Vector Description Microscopy Image Comments""" KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS RFP and CFP signal KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS RFP and CFP signal KAH54 PcVN [[K : Gal4 DB] + VP64-NLS CFP signal only KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS RFP signal only KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS RFP and CFP signal Since RFP and CFP expressed at the same time, I concluded that both plasmids transfected to the U2OS cells and VP64 activated CFP expression. KAH54 has no mCherry sequence but VP64 could activate CFP expression and KAH59(MV5) without VP64 only shows RFP signal.