User:Beatriz Gimenez De C./Notebook/571/2014/11/12

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Tasks

  • Polarization Fluorescence
    • Prepare 10 μM tryptophan solution
      • measure fluorescence with excitement 280 nm + collect data between 300 and 550 nm
    • measure 10 μM lysozyme, 10 μM HRP and 10 μM Lipase using no filter, vertical emission filter, and horizontal emission filter
    • Plot fluorescence intensities vs. molar mass of proteins/tryptophan
    • Plot (vert - horiz)/(vert + 2*horiz) vs. molar mass of proteins/tryptophan
  • Create calibration curve for CaCl2
CaCl2 Concentration Potential measurement (mV)
0.05 5.2
0.5 35.6
5 63.7
15 75.6
25 81.7
35 84.0
45 87.1

Image:CaCl2 calibration.png


Personal tools