User:Beatriz Gimenez De C./Notebook/571/2014/10/08

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Tasks

  • Prepare 50 mL 50 mM KI Stock Solution (store in 50 mL Falcon tube) (used 0.2075 g)
  • Prepare 50 mL 75 mM AgNO3 Solution
    • added 0.6370 g AgNO3 measured with Rm 205 balance
    • Store in Black 50 mL Falcon tube
  • Prepare 100 mL 10 mM NH4SCN (0.0761g of ammonium thiocyanate) Stock Solution (store in Falcon tubes)
  • NH4SCN Standardization
    1. Add 3 mL 1 M HNO3, 2 mL AgNO3 to 10 mL DI H2O and 200 μL Ferric alum indicator
    2. Titrate with your NH4SCN solution
    3. solution turned orange at 15.5 mL for both trials
  • KI Standardization
    1. Add 3 mL 1 M HNO3 and 1 mL KI to 10 mL DI H2O
    2. Add 2 mL AgNO3 and swirl to complete precipitation
    3. Observed yellow precipitate
    4. Add 200 μL Ferric alum indicator
    5. Titrate with your NH4SCN solution, turned orange at 10.3 mL in the first trial and 10.5 mL in the second trial.
  • [I-] for dialysis
    • Set up Ki dilution agains 0.6 g/L lysozyme using MWCO 3500
      • 50 mM, 25 mM, 10 mM, 5mM and 2 mM KI solutions were set aGAINST 0.6 g/L lysoszyme.


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