User:Beatriz Gimenez De C./Notebook/571/2014/10/07

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Tasks

  • Prepare 0.6g/L lysozyme stock
    • 42 μM = 0.6 g/L Lysozyme (add 0.015 g Lysozyme to 25 mL deionized water)
    • 30:1 lysozyme-gold colloid
      • Add 0.5 mL 250 mM HAuCl4 , 1 mL 42 μM (0.6 g/L) Lysozyme, and 3.5 mL HPLC-grade H2O to 5 culture tubes
      • Place in oven for 4 hours
    • 50 mm NaCl 50 mM Tris buffer solution was made by adding:
      • 0.3030g of Tris buffer
      • 0.1461g of NaCl
      • Add to 50 mL distilled water
  • Bradford Analysis
    • Solutions were prepared on October 01
      • 200 μL Bradford Reagent (diluted 1:3 with Tris buffer)
      • 780 μL 50 mM Tris/ 50 mM NaCl Buffe ( Solutions were vortexed in a 1.5 mL centrifuge tube and equilibrated for 5 minutes)
      • Ran UV-Vis between 400-800 nm using PS cuvettes
      • An Undialyzed Lysozyme Bradford analysis was also ran.
  • Measured pH of H+ containing solutions
Sample pH
0.25 mM HCl (4) 8.062
Lysozyme (4) 8.134
  • Performed Ca2+ ISE of Ca2+ containing solutions
Substance Potential (mV)
Colloid Sample 1 73.5
50 mM CaCl2 (1) 74.9
Colloid Sample 2 51.2
500 μM CaCl2 91.4



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