Tasks
- Prepare 0.6g/L lysozyme stock
- 42 μM = 0.6 g/L Lysozyme (add 0.015 g Lysozyme to 25 mL deionized water)
- 30:1 lysozyme-gold colloid
- Add 0.5 mL 250 mM HAuCl4 , 1 mL 42 μM (0.6 g/L) Lysozyme, and 3.5 mL HPLC-grade H2O to 5 culture tubes
- Place in oven for 4 hours
- 50 mm NaCl 50 mM Tris buffer solution was made by adding:
- 0.3030g of Tris buffer
- 0.1461g of NaCl
- Add to 50 mL distilled water
- Bradford Analysis
- Solutions were prepared on October 01
- 200 μL Bradford Reagent (diluted 1:3 with Tris buffer)
- 780 μL 50 mM Tris/ 50 mM NaCl Buffe ( Solutions were vortexed in a 1.5 mL centrifuge tube and equilibrated for 5 minutes)
- Ran UV-Vis between 400-800 nm using PS cuvettes
- An Undialyzed Lysozyme Bradford analysis was also ran.
- Measured pH of H+ containing solutions
| Sample
|
pH
|
| 0.25 mM HCl (4) |
8.062
|
| Lysozyme (4) |
8.134
|
- Performed Ca2+ ISE of Ca2+ containing solutions
| Substance
|
Potential (mV)
|
| Colloid Sample 1 |
73.5
|
| 50 mM CaCl2 (1) |
74.9
|
| Colloid Sample 2 |
51.2
|
| 500 μM CaCl2 |
91.4
|
|
Project name
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