User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/24

1. Induce cells if necessary and lyse by the end of the day (or the next morning)

Primer design information

>wt TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA >mutant TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTTA

wt16sProbe1

• 5'-GGT TGG ATC ACC TCC-3' 49

wt16sProbe2

• 5'-GTT GGA TCA CCT CCT TA-3' 48 - ordered this

o16sProbe1

• 5'-GTT GGA TCA TTG TGG-3' 44

o16sProbe2

• 5'-GTT GGA TCA TTG TGG TTA-3' 47 - ordered this