We the laboratory of nanotechnology and chemical biology (LNCB) is part of Regional Centre for Biotechnology, established by Department of Biotechnology, Govt of India; that is part of Biotech-Cluster Faridabad, having THSTI, NIPGR, NII its members.
Our research group is working in the area of Bio-nanotechnology including Nanotechnology and Cancer Biology. We will be using interdisciplinary approach including synthetic chemistry, cancer biology, pharmacology, and bioinformatics. Our research interests include drug delivery, gene therapy, combination therapy for cancer therapy and exploiting the nucleo-cytoplasmic communications to find new targets for cancer nanomedicine.
Nanomedicine: Engineering of hybrid nanoparticles for effective, targeted, and controlled drug/gene delivery is one of the major challenges for successful cancer therapy. The design of new materials to overcome the existing limitations of delivery vehicles, and to target multiple pathways is required for successful cancer therapy.
Our research groups focuses on engineering of different nanomaterials based on liposomes, polymer, dendrimers and nanoparticles as effective drug delivery systems for cancer therapy. One of the major challenges in cancer therapy is the lack of universal drug delivery systems, as cure for cancer depends strongly upon the genetic background of the cancer cells. We are exploiting how the genetic background of the cancer affects the drug delivery systems in cancer therapy.
As various cellular signaling pathways regulate the cell proliferation, invasion, and metastasis of cancer cells, it is challenging to develop cellular toxicity by cutting down only one of the pathway. Therefore, the combination therapy and especially the combination of chemotherapy and gene therapy can be one of the strategies to cut down the multiple cellular pathways for effective cancer therapy. We are developing nanomaterials comprising drug delivery and DNA/siRNA delivery vehicles that would transform the cancer therapy by increasing efficacy of cutting down multiple pathways.
Cancer Biology: The understanding of nucleo-cytoplasmic communications in cancer cells is required for development and engineering of effective cancer therapies. Therefore, we are exploiting these communications in colon, lung, and breast cancer models to understand the common targets in different tissue specific tumors. We would be using different genomic, metabolomic and proteomic approaches to discover different molecular targets for cancer therapy in tissue specific tumors.
1. S. Rana, A. Singla, A. Bajaj, S. G. Elci, O. R. Miranda, R. Mout, B. Yan, F. R. Jirik, V. M. Rotello, Array-Based Sensing of Metastases and Metastatic Cells and Tissues Using Nanoparticle-Fluorescent Protein Conjugates" ACS Nano 2012,
2. C. Subramani, K. Saha, B. Creran, Avinash Bajaj, D. F. Moyano, H. Wang, and Vincent M. Rotello, Cell alignment using patterned biocompatible gold nanoparticle templates, Small, 2012, 8, 1209-1213.
3. Subinoy Rana, Avinash Bajaj, Rubul Mout, Vincent M. Rotello, Monolayer coated gold nanoparticles for delivery applications, Advanced Drug Delivery Reviews, 2012, 64, 200-216.
4. Krishnendu Saha, Avinash Bajaj, Bradley Duncan, and Vincent M. Rotello, Beauty is skin deep: a surface monolayer perspective on nanoparticle interactions with cells and biomacromolecules, Small, 2011, 7, 1903-1918.
5. C. Subramani, N. Cengiz, K. Saha, T. N. Gevrek, X. Yu, U. Jeong, Avinash Bajaj, A. Sanyal, and Vincent M. Rotello, Direct fabrication of functional and biofunctional nanostructures through reactive imprinting, Adv. Mater. 2011, 23, 3165-3169.
6. Joydeep Biswas, Avinash Bajaj, and Santanu Bhattacharya, Aggregation and DNA Binding Properties of Hydroxylated and Non-Hydroxylated Cholesterol Based Gemini Lipids and Their Interaction with DPPC Membranes” J. Phys. Chem. B, 2011, 115, 478-486.
7. C. Subramani,* Avinash Bajaj,* O. R. Miranda and V. M. Rotello Anti-Protein Fouling Properties of Gold Nanoparticles on Surfaces. Adv. Mater. 2010, 22, 5420-5423. (* equal contribution)
8. Avinash Bajaj, S. Rana, O. R. Miranda, I. B. Kim, R. L. Phillips, D. J. Jerry, U. H. F. Bunz, V. M. Rotello, Cell surface-based differentiation of cell types and cancer states using a gold nanoparticle-GFP based sensing array. Chem. Sci. 2010, 1, 134-138.
9. Avinash Bajaj, O. R. Miranda, I. B. Kim, R. L. Phillips, D. J. Jerry, U. H. F. Bunz, V. M. Rotello, Array based sensing of mammalian cell types using conjugated polymers.” J. Am. Chem. Soc. 2010, 132, 1018-1022.
10. Avinash Bajaj, O. R. Miranda, Ik-bum Kim, Ronnie L. Phillips, D. Joseph Jerry, Uwe H. F. Bunz and Vincent M. Rotello, Patterned array based detection of normal/cancerous/metastatic cells using nanoparticle-polymer supramolecular complexes. Proc. Nat. Acad. Sci. USA 2009, 106, 10912-10916.
11. Avinash Bajaj, B. Samanta, Haoheng Yan, D. Joseph Jerry, Vincent M. Rotello, Stability, toxicity and differential cellular uptake of protein passivated-Fe3O4 nanoparticles. J. Mater. Chem. 2009, 19, 6328-6331.
12. Melissa D. Krebs, Randall M. Erb, Benjamin B. Yellen, Bappaditya Samanta, Avinash Bajaj, Vincent M. Rotello, Eben Alsberg, Formation of ordered cellular structures in suspension via label-free negative magnetophoresis. Nano Letters 2009, 9, 1812-1817.
13. Santanu Bhattacharya and Avinash Bajaj, Recent advances in gene delivery through molecular design of cationic lipids. Chem. Commun. 2009, 6432-6456.
14. Avinash Bajaj, Paturu Kondaiah and Santanu Bhattacharya, Gene transfection efficacies of novel cationic gemini lipids possessing aromatic backbone and oxyethylene spacers. Biomacromolecules 2008, 9, 991-999.
15. Avinash Bajaj, Santosh K. Mishra, Paturu Kondaiah and Santanu Bhattacharya, Effect of the head groups on gene transfection efficiencies of cholesterol based cationic lipids. Biochim. Biophys. Acta: Biomembranes 2008, 1778, 1222-1236.
16. Avinash Bajaj, Paturu Kondaiah and Santanu Bhattacharya, Effect of the nature of spacer on gene transfer efficacies of thiocholesterol derived gemini lipids in different cell lines: A structure-activity investigation. J. Med. Chem. 2008, 51, 2533-2540.
17. Avinash Bajaj, Bishwajit Paul, Paturu Kondaiah and Santanu Bhattacharya, Structure-activity investigation on the gene transfection efficiencies of cariolipin mimicking gemini lipid analogues. Bioconjugate Chem. 2008, 19, 1283-1300.
18. Santanu Bhattacharya and Avinash Bajaj, Fluorescence and thermotropic studies of the interactions of PEI-cholesterol based lipopolymers with dipalmitoylphosphatidylcholine (DPPC) membranes. Biochim. Biophys. Acta: Biomembranes 2008, 1778, 2225-2233.
19. Avinash Bajaj, Paturu Kondaiah and Santanu Bhattacharya, Synthesis and gene transfection properties of PEI-cholesterol based lipopolymers. Bioconjugate Chem. 2008, 19, 1640-1651.
20. Santanu Bhattacharya and Avinash Bajaj, Membrane forming properties of pseudoglyceryl backbone based gemini lipids possessing oxyethylene spacers. J. Phys. Chem. B 2007, 111, 2463-2472.
21. Avinash Bajaj, Paturu Kondaiah and Santanu Bhattacharya, Synthesis and gene transfection properties of novel serum compatible cholesterol based gemini lipids possessing oxyethylene spacers. Bioconjugate Chem. 2007, 18, 1537-1546.
22. Avinash Bajaj, Paturu Kondiah and Santanu Bhattacharya, Design, synthesis and in vitro gene transfection properties of novel cholesterol based gemini lipids and their serum compatibility: A structure-activity investigation. J. Med. Chem. 2007, 50, 2432-2442.
23. Santanu Bhattacharya and Avinash Bajaj, Thermotropic and hydration studies of gemini pseudoglyceryl lipids possessing polymethylene spacers. Langmuir 2007, 23, 8988-8994.
24. Avinash Bajaj, Bishwajit Paul, S. S. Indi, Paturu Kondaiah and Santanu Bhattacharya, Effect of the hydrocarbon chain lengths and spacer lengths on gene transfection efficacies of gemini lipids based on aromatic backbone. Bioconjugate Chem. 2007, 18, 2144-2158.
25. Santanu Bhattacharya and Avinash Bajaj, Membrane-forming properties of aromatic derived gemini lipids possessing polymethylene spacers. J. Phys. Chem. B, 2007, 111, 13511-13519.
26. Bishwajit Paul, Avinash Bajaj, S. S. Indi and Santanu Bhattacharya, Synthesis of novel dimeric lipids based on an aromatic backbone between the hydrocarbon chains and head groups. Tetrahedron Lett. 2006, 47, 8401-8405.
27. Santanu Bhattacharya and Avinash Bajaj, Recent trends in lipid molecular design. Curr. Opin. Chem. Biol. 2005, 9, 647-655.
28. Avinash Bajaj, Subinoy Rana, Oscar Miranda, Uwe H. F. Bunz and Vincent M. Rotello, “Detection of normal/cancerous/metastatic cells using nanoparticle polymer and nanoparticle-GFP supramolecular complexes” 2009, Applied for US Patent (UMass-Amherst docket number UMA 09-40).