User:Asya L. Tucker/Notebook/Asya 571/2015/12/01

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Objective

As a class we developed standard procedure for running ICP, conductivity and UV-Vis experiments for the following day and prepared the respective samples.

Procedure

1. Dr. Hartings prepared 20 mL AU fibers for the class. Procedure Here

2. We made the following samples

Sample Concentration (of Trypsin) Amount Buffer Amount of Trypsin
11.0 uM19.7425 mL0.2575 mL of 77.68 uM
21.0 uM19.7425 mL 0.2575 mL of 77.68 uM
31.0 uM19.7425 mL0.2575 mL of 77.68 uM
40.1 uM19.9742 mL 0.02575 mL of 77.68 uM
50.1 uM19.9742 mL 0.02575 mL of 77.68 uM
60.1 uM19.9742 mL 0.02575 mL of 77.68 uM
70.01 uM19.99 mL 0.01 mL of 1.0 uM
80.01 uM19.99 mL0.01 mL of 1.0 uM
90.01 uM19.99 mL 0.01 mL of 1.0 uM
100.001 uM19.999 mL 0.001 mL of 1.0 uM
110.001 uM19.999 mL0.001 mL of 1.0 uM
120.001 uM19.999 mL0.001 mL of 1.0 uM
Supernatant
1 no Trypsin00
2 no Trypsin00
3no Trypsin00

3. We incubated all samples in a 37 degree C water bath for approximately 21 hours.


Personal tools