User:Ashlin Rakhra/Notebook/Biology 210 at AU

Lab 6 Embryology= sequence of events following fertilization, growth, differentiation, morphogensis 2 groups --> protosomes & deuterostomes Selective Pressures --> food and development time

H2O= 20 embryos in egg case Caffeine= 3 hatchlings and 17 embryos in egg cases

Lab 5 Invertebrates 3 germ layers --> ecto, meso & endoderm

3 stations 1. Planaria- acoelomates- eating egg yolk 2. Nematodes- pseudocoelomates- eating bacteria 3. Annelids- coelomates

Lab 4 Types of plants Non vascular plants (Bryophytes, mosses) Seedless plants (ferns) Gymnosperms seed plants Anngiosperms seed plants

Vegative structures- roots, stems, leaves Reproductive structures- flowers, fruits, seeds Fungi: Sacrophytic- derives nourishment from dead matter

Briefly describe the vascularization in each of the plants from your transect: Monocot, vascularization, 24 inches or 8.5 cm

Lab 3 The tetracycline, for the most part, killed the bacteria.

Table 1 100-fold Serial Dilution Results Dilution Agar Colonies Counted Conversion Factor Colonies/ml 10^-3 nutrient 120 x10^3 120,000 10^-5 nutrient 30 x10^5 3,000,000 10^-7 nutrient 4 x10^7 40,000,000 10^-9 nutrient 17 x10^9 1.7x10^10 10^-3 nutrient+tent 23 x10^3 23,000 10^-5 nutrient+tent 1 x10^5 100,000 10^-7 nutrient+tent 1 x10^7 10,000,000

Table 2: Gram Staining= classical method for identifying bacteria Colony Label Tet or NA Colony Description # of colonies Cell Description Gram pos/neg 10^-5 NA dark purple,circular, margin (entire) 30,000,000 Diplobacilli, fast, motility, cilia neg 10^-5 Tet circular, cream color, flat, convex 1,000,000 Browian movement neg 10^-3 Tet small, yellow circular, convex 230,000 Streptobacilli, no movement pos

1/27 Lab 1:' The objectives of Lab 1 were to: 1. understand natural selection 2. Understand the biotic and abiotic characteristics of a niche

Notes from powerpoint: Classification- Kingdom, phylum, class, order, family, genus, species Volvocine Line: example of evolution Chlamydomonas- single cell Gonium- multicellular (4-32 cells) Volvox- ball of cells, hollow sphere

Evolution- genetic change in populations over generations Natural Selection- mechanism controlling differential reproduction of phenotypes causing traits

The Volivicine Line (procedure 1)

Table 1 Evolutionary Specialization of Members of the Volvocine line Charactersitic Chlamydomonas Gonium Volvox Number of cells: 1 8 11 (Balls of cells) Colony size: large small small Describe any functional specialization: random movement slower movement hardly any movement Describe any reproductive specialization: isogamy oogamy oogamy

Notes from Transect 1 my group observed the transect and found: (Procedure 2) Biotic- grass, weeds, red bush, cat tail bush Abiotic- wood chips, big rocks, little rocks, soil, pebbles dead leaves, brick File:/var/folders/f8/wqm4h0x134vdmmsz1rv3trbr0000gn/T/Messages/Transfers/IMG 7866.jpeg]] File:/var/folders/f8/wqm4h0x134vdmmsz1rv3trbr0000gn/T/Messages/Transfers/IMG 2473.jpeg We also made a Hay infusion

Lab2: 7 key lineages of protists: 1. Amoeboza- engulf food, fresh water ex. cellular slime mold 2. Excavata- have excavated folding groove ex. euglenids 3. Plantae- plankton, flagella ex. green algae 4. Rhizaria- amoebae lacking cell walls ex. plankton 5. Alveolata ex. ciliates 6. Stramenopila

4 Trends: unicellular/ multicellular; cell specialization; modes of nutrion- photosynthesis, saprophytic, ingest; asexual to sexual reproduction

Findings: Slide 1: Multicellular invertebrate (contaminated worm like "creeping" around) & paramecium (twisted)

Hay infusion: smell- peas, stinks cloudy water, plants floating, brown algae on bottom and on side of jar, algae on top of water 1. specimen of brown algae from top layer of surface 2. Moving figure (possible Didinium Cyst) 3. Chlamydomonas (2.5 um)

from bottom layer 1. spirostomum (2um) 2. brown algae 3. Colpidium (62um)

Good start. Could include more detailed description of work undertaken and why. Address all red text in the protocol and include headings for sections for example: Conclusions. For more instructions see TA notebook. SK

1/23/14

I have successfully entered data into my lab notebook.