User:Anthony Salvagno/Notebook/Research/2010/12/20/New Anchor PCR Construct Design
Initial work didn't go so well as Primer3 couldn't find suitable primer pairs, but I think I found a decent pair manually.
- GGATCTCAACAGCGGTAAG - located between 701 and 751. It is 19bp long and melts at like 61C.
- CTTCCAGATCGTTGGACAG - located just beyond 201. It too is 19bp long and melts at 61C.
Total product length equals 4087bp.