User:Anthony Salvagno/Notebook/Research/2010/06/25/Unzipping Stuff:Gel Extraction and Gel analysis
See these notes. 1mg/ml is my stock of Neutravidin and I am starting with 10um .025 dsDNA with overhangs. According to my notes the stock is ~16uM so I can do a 1:1 reaction and then run a gel.
Gel of pBR Digest
I also gel extracted the wrong piece piece of DNA so I will have to run a diagnostic gel of the gel extracted stuff to make sure I'm right. I will then simultaneously gel extract the piece that I messed up in case I am right. Fun in the sun.
I am determined to finish the ligations today (tomorrow) so once the diagnostic checks out I will be doing this reaction. If it fails then it will have to wait.