User:Anthony Salvagno/Notebook/Research/2010/05/24/First Attempt at Unzipping of the New Era
I start by cleaning glass:
- Sonicator with water and alconox for 480 seconds
- Rinse slides
- Air dry
I cleaned 4 slides and 4 coverslips and made flow cells out of them. I have an old bunch of anti-dig and BGB so I will make more of that. For the BGB I have already made, but not yet purified stuff so I will purify that into an eppi. The anti-dig gets diluted 1:10 in PBS. Next I prepare a sample of beads by diluting 1:20 in BGB (for bead samples I use water) and then I sonicate for ~15s.
Then I tether:
Other Version of Tethering
It looks pretty cool, but I don't know how to get the checks to be permanent. I'll stick with my spreadsheet for now.