User:Anthony Salvagno/Notebook/Research/2010/05/17/Tweezer Operations with Pranav
We started off with a sample of beads. I showed Pranav the exact method of making a sample of beads:
- prepare a sample chamber
- dilute beads 1:20
- sonicate beads for 15 seconds
- dilute beads again (first try we did another 1:20 but later I did 1:100)
- flow 10ul BGB
- wait 30 seconds
- flow 10ul diluted beads
- seal chamber
Then we tried to get some power spectrum data. We were getting all kinds of noise and no matter what we tried we had various frequency interruption. I then got a little experience with visual sourcesave and updated our live feed software. Next I got a little experience with labview and altered the software to my specifications. Larry amended the software to fit Andy's needs (his camera is rotated 90 degrees and mine isn't), so I changed the parameter that rotates the image so that there is no rotation. It was a real easy fix, but I did it with relatively no labview experience so I'm proud. We also cleaned some dirt off the camera ccd.
With all that taken care of we made a sample of stuck beads. I don't know why I thought this would work, but I did and it didn't. We kept getting a linear power spectrum and couldn't figure out why. Finally I realized it is because the beads need to be moving and so we captured moving beads. Next I couldn't get the power spectrum program to fit the plot and I realized that it was taking into account all the data below 20hz as well. It took me a while, but I figured out how to remove those points from the data and then I got a great fit. So today (because I am writing this late) Pranav and I will get all the stuff done that we were supposed to do yesterday. See tomorrow's notes for updates.