User:Anthony Salvagno/Notebook/Research/2010/02/17/Tethering with Tweezers?
We may have tweezing... yay!
- Clean glass: 180sec in sonicator, rinsed thoroughly, blow dried
- bead prep: 1:25 dilution in BGB
- DNA: pBR322 diluted 1:10 from gel extracted amount
Tethering was pretty smooth. There were tons of tethers although at first, there wasn't that many. The solution of beads was a little more clumpy than I had hoped for, but I guess I should sonicate a little more next time. I tried unzipping, but I don't really understand the data yet so who knows what I got.