User:Anthony Salvagno/Notebook/Research/2010/01/05/Preplanning: Shotgun DNA Mapping Tethers, the SapCap Returns
It is finally time to get this poster ball rolling. With the arrival of the AOM days away, the new detector, and finally the Shotgun clones I can begin the
final second-to-last phase of this project. Sadly I am at the point I was a year ago, but this time I have had much more success after a quiet summer. I will need to prepare the clones for some ligation to the construct, and then I will ligate them to the construct. Then (if it all works on the 1st try which is not likely to happen) in a few days I can tether and try my hand at unzipping. But first let's plan this thing out:
Determining Molarity of Shotgun Fragments
This is what I must do first.
It seems like I never purified the DNA after Sap digestion and I am getting really funky numbers (ie: SapD=1287ng/ul). I just checked and the results are inconclusive, but the samples smell not like elution buffer so I'm guessing I'm right. Also my lack of notebooking skills in my first 3 months of going public really suck and I leave out a lot of crucial information. I have learned a lot in a year.
Anyways, I will purify the fragments and then try this part again.
Ligation of Sap Cap Plan
So I failed on this thing, but I will work on this again tomorrow.