User:Anthony Salvagno/Notebook/Research/2009/10/27/Redoing Unzipping: Anchor PCR

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1st Things 1st

I am going to start by remaking the stocks of the primers cause they are old and possibly contaminated. Its a shame really cause Brandon wrote so nicely on the top of the tubes. It is labeled perfectly and everything fits and is nice and neat. Sigh.

R1985 is 69uM
F834-dig is 97uM
I am making 500uL of 10uM stocks so 72.5ul of R1985 and 51.4ul of F834-dig suspended in 1x TE buffer.

Recipe

Results

This is depressing.