User:Anthony Salvagno/Notebook/Research/2009/07/14/PCR to make dsDNA for stretching

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The purpose of today's exercise is to hopefully create some tetherable dsDNA for stretching experiments. The procedure I followed is from a previous experiment of Koch's that he performed with Diego Ramallo seen here.

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Thermal Cycler Program

Stage Temp (C) Time (mm:ss) Reps
1 94 4:00 1
2 94 1:00
60 1:00
72 5:00 35
3 72 9:00 1
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