User:Anthony Salvagno/Notebook/Research/2009/04/17/BstXI Digestion and PCR Results
After returning from slumber I P/C extracted-EtOH precipitated the PCR-ed DNA to clean it up. Nanodrop readings provided 989.9ng/uL. So yup I have 50ug of DNA. Sweet.
Since the PCR went smoothly, we are going to digest the new DNA with BstXI to create an overhang so that we can ligate a biotinylated oligo and have our anchor construct.
Let that run for 4 hours. On Monday I will need to run a gel and gel purify to remove the short (40bp) fragments created from this digestion. This reminds me. I will need to upload an image of this process here with a sequence map of this piece and all the other pieces that I will be working with.
Anthony Salvagno 17:50, 17 April 2009 (EDT):Koch can you help with this?