User:Anthony Salvagno/Notebook/Research/2009/02/05/Proteinase K Buffer Protocol

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This is a new solution (for me) that we made to STOP the lyticase enzyme from completely destroying the cells. Call it PK for short

Procedure

Need:

  • 50mM EDTA
  • 0.3% SDS
  • 0.01% Proteinase K

To make 25mL:

  1. from 500mM EDTA take 2.5mL
  2. from 10% SDS take .75mL
  3. from 21 mg/mL stock of Proteinase K take 119uL (need 2.5mg)
  4. mix
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