User:Alicia Rasines Mazo/Notebook/CHEM-581 Experimental Chemistry I/2014/10/24

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Tasks for October 24 To prepare packed columns To perform column chromatography on BPA solutions from Oct. 22 Preparation of packed column Place glass wool on bottom of column Cover with a layer of sand Add silica packing material prepared by dissolved 10 g of silica in methanol Allow to settle Rinse with water to remove ethanol Concentration of 10.4 ppm BPA solution Added 10 mL of BPA Added 10 mL water Added 30 mL of methanol Collected eluate at 5 mL intervals Spotted on TLC plate, and using UV light, checked for fluorescence. Water 2, Methanol 1 and methanol 2 culture tubes gloved faintly.