User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/13

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Contents

PCR Clean Up and Cycle Seq S. cerevisiae

CS Reaction mixture conc. 1x 4
Big Dye Terminator0.52
DMSO0.10.4
5X Seq Buffer5X0.40.8
Primer (FWD or RVS)5uM0.41.6
Template *~1-3ng/ul4.6
2.0ul / RXN
*QIAquick Cleaned PCR product
Cycle Seq Program
96C10 sec
50C5 sec
60C4 min
x60
MATA2 FWD and RVS
GenusspeciesNCYC #sample
Saccharomycescerevisiae6089318
Saccharomycescerevisiae6089419
Saccharomycescerevisiae6089520
Saccharomycescerevisiae6089621
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