User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/04

Alien genes

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Inoculated Liquid culture with yeast from slants NCYC 2827, 2878, 415, 971 and 814 1) 10mL Glucose+Yeast Media broth with sterile toothpick 2) Incubate at 30C QIAquick PCR purification and Cycle Sequencing Clean S. Dairenenses with QIAquick 4.9ng/ul CS Reaction mixture conc. 1x 2 Big Dye Terminator 0.5 1 DMSO 0.1 0.2 5X Seq Buffer 5X 0.4 0.8 Primer (FWD or RVS) 5uM 0.4 Template * ~1-3ng/ul 4.6 2.0ul / RXN *QIAquick Cleaned PCR product Cycle Seq Program 96C 10 sec 50C 5 sec 60C 4 min x60 MATA2 FWD and RVS Genus species NCYC # sample Saccharomyces dairenensis 777 4