User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/04

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Contents

Inoculated Liquid culture with yeast from slants

NCYC 2827, 2878, 415, 971 and 814

1) 10mL Glucose+Yeast Media broth with sterile toothpick 2) Incubate at 30C


QIAquick PCR purification and Cycle Sequencing

Clean S. Dairenenses with QIAquick 4.9ng/ul

CS Reaction mixture conc. 1x 2
Big Dye Terminator0.51
DMSO0.10.2
5X Seq Buffer5X0.40.8
Primer (FWD or RVS)5uM0.4
Template *~1-3ng/ul4.6
2.0ul / RXN
*QIAquick Cleaned PCR product
Cycle Seq Program
96C10 sec
50C5 sec
60C4 min
x60
MATA2 FWD and RVS
GenusspeciesNCYC #sample
Saccharomycesdairenensis7774
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