User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/23

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Contents

MATA2 Amplification on Chelex Extraction

EX Takara RXN Mixture conc 1X 3
Milli Q water6.1518.45
Takara EX Buffer10X13
dNTP2.5uM0.82.4
Primer Mix5uM13
EX Taq5U0.050.15
TemplateChelex Extraction1
27
9.0ul / RXN and 1.0ul Chelex Extraction
GenusspeciesNCYC #sample
Schizosaccharomycespombe27221
Saccharomycescerevisiae10222
PCR cycle
98C10 sec
60C1 min

Load 2.0ul


Image:23July2010.jpg

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