Adenita Alien Gene LongPCR
Re-Amp 19 and 21
| '
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[CONC]
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1X
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6
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'
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'
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AMP Lane
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Template
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MIX
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Contents
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'
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Size
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Fosmid
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| Water |
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10.6 |
63.6 |
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19 |
CCA3 |
111 |
AV10106 FWD |
AV10108.2 RVS |
13400 |
AV_TEL_L_B
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| Takara LA Taq |
5U |
0.2 |
1.2 |
|
|
21 |
CCA3 |
55 |
AV10108 FWD |
AV10111 RVS |
14846 |
AV_TEL_L_B
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| Takara LA Buffer |
10X |
2 |
12 |
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CCA3 |
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| dNTP |
2.5uM each |
3.2 |
19.2 |
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| *Template |
3.8ng |
2 |
12 |
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| Primer Mix |
5uM each |
2 |
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10.0ul |
108 |
18.0ul/RXN |
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add 2.0 Primer Mix |
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| * CCA3 |
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| PCR Cycle |
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| 94C |
1 min |
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| 98C |
10 sec |
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| 65C |
15 min |
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30X
|
load 2.0 ul of sample
Library preparation for Alien genes
Fragmentation check by bioanalyzer
| lane
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Sample
|
conc.
|
used
|
| 1 |
AGM end repaired |
10 ng/ul |
1 ul
|
| 2 |
AGM 1day ligaiton |
33.8 ng/ul |
1 ul
|
| 3 |
AGM 2days ligaiton |
21.9 ng/ul |
1 ul
|
| 4 |
AGM 3days ligaiton |
31.0 ug/ul |
1 ul
|
|
Alien genes
Main project page
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