User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/21

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Contents

Adenita Culture 454 Amplification Adapter extension

EX Takara RXN Mixture conc 1X 16 ' '
Milli Q water6.1598.4
Takara EX Buffer10X116
dNTP2.5uM0.812.8
Primer Mix20uM1
EX Taq5U0.050.8
TemplateOleg PCR 1:101
PCR 1:5128
8.0ul / RXN add 1.0ul Primer and 1.0ul PCR template
PCR cycle
98C10 sec
60C1 min
x15
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
1Culture 11 1:5B111Culture 11 1:10B11
2Culture 11 1:5D112Culture 11 1:10D11
3Culture 11 1:5E113Culture 11 1:10E11
4Culture 11 1:5F114Culture 11 1:10F11
5Culture H 1:5B125Culture H 1:10B12
6Culture H 1:5C126Culture H 1:10C12
7Culture H 1:5E127Culture H 1:10E12
8Culture H 1:5F128Culture H 1:10F12

Load 2.0ul of sample


Image:21June10.jpg

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