User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/09

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HCO LCO Cycle Sequencing Purification with QIAquick

For Culture 14 - failed injection

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2

    • see page 19.
    • 6ul product for cleaning. Resuspended in 10ul EB buffer.

Alien Gene PCR Purification with QIAquick

For previously Ethanol Cleaned samples 09April10

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2

    • see page 19.
    • ~8ul PCR product left for cleaning. Resuspended in 30ul EB buffer.
Sample ID Nucleic Acid Conc. Unit A260 A280 260/280 260/230 Sample Type Factor
114.5ng/µl0.290.1491.951.68DNA50
212.3ng/µl0.2450.1222.012.21DNA50
37.7ng/µl0.1530.0642.41.81DNA50
416.3ng/µl0.3270.171.922.27DNA50
511.4ng/µl0.2280.1092.091.79DNA50
615ng/µl0.30.1521.971.83DNA50
77.5ng/µl0.1490.0672.242.03DNA50
834.8ng/µl0.6970.3851.812.01DNA50
930.8ng/µl0.6170.3411.811.99DNA50
105.8ng/µl0.1160.0621.861.74DNA50
1114.2ng/µl0.2850.1471.931.83DNA50
1210.9ng/µl0.2190.1022.151.86DNA50
138.9ng/µl0.1790.0941.912.32DNA50
1412.9ng/µl0.2580.1361.892.05DNA50
1544.4ng/µl0.8890.4891.821.8DNA50
1611.1ng/µl0.2220.1281.731.63DNA50
1711.5ng/µl0.230.1191.922.21DNA50
1811ng/µl0.2210.1072.072.17DNA50
196.1ng/µl0.1220.0611.991.71DNA50
206.4ng/µl0.1280.0622.082DNA50
215.4ng/µl0.1090.0671.631.56DNA50
2210.2ng/µl0.2040.1211.681.96DNA50
2315.3ng/µl0.3060.1751.751.56DNA50
2415.3ng/µl0.3060.1641.871.57DNA50
2510.3ng/µl0.2060.12.051.91DNA50

Sample to add to mix

Sample MIX Contents ' Size Gene CCA3 Result Qiagen Cleaned ng/ul Sample Type Total Product Remaining bp/ng ul to mix ng added ul to mix x 5
123AV10004 FWDAV10007 RVS8691AV212 Agood 04Mar1014.5DNAx 29ul0.0016683931.09140710215.825402995.457035512
21AV10007 FWDAV10011 RVS9262AV212 Agood 04Mar1012.3DNAx 29ul0.0013280071.37114913216.865134336.855745662
32AV10011 FWDAV10016 RVS11924AV212 Agood 02Mar107.7DNAx 29ul0.0006457562.8197867821.7123582114.0989339
424AV10016 FWDAV10020 RVS7020AV212 Agood 04Mar1016.3DNAx 29ul0.0023219370.784213912.782686573.921069499
525AV10020 FWDAV10025 RVS11202AV212 Agood 04Mar1011.4DNAx 29ul0.0010176751.78926944220.397671648.946347211
64AV10025 FWDAV10028 RVS6843AV212 Agood 04Mar1015DNAx 29ul0.0021920210.83069253712.460388064.153462687
734AV10029 FWDAV10034 RVS12093AV240 Bgood 08Mar107.5DNAx 29ul0.0006201942.9360119422.0200895514.6800597
840AV10034 FWDAV10039 RVS14180AV240 Bgood 18Mar1034.8DNAx 29ul0.0024541610.74196260125.820298513.709813004
950AV10039 FWDAV10041 FWD19555AV240 Bgood 18Mar1030.8DNAx 29ul0.0015750451.15609129735.607611945.780456484
1030EU643474 FWDEU643474 RVS10062AV240 Bgood 04Mar105.8DNAx 29ul0.0005764263.15893978418.3218507515.79469892
1131AV10045 FWDAV10047 RVS6762AV240 Bgood 04Mar1014.2DNAx 29ul0.002099970.86710531812.312895524.335526592
1232AV10047 FWDAV10049 RVS12719AV240 Bgood 04Mar1010.9DNAx 29ul0.0008569862.12476790423.1599701510.62383952
1333AV10049 FWDAV10053 RVS9353AV240 Bgood 04Mar108.9DNAx 29ul0.0009515661.91357705917.030835829.567885293
1478AV10091 FWDAV10092.2 RVS8127AV TEL L Agood 19Mar1012.9DNAx 29ul0.0015873021.14716417914.798417915.735820896
1585AV10092.2 FWDAV10094 RVS7036AV TEL L Agood 01Apr44.4DNAx 29ul0.0063104040.28855452512.81182091.442772623
1616AV10094 FWDAV10098 RVS8600AV TEL L Agood 02Mar1011.1DNAx 29ul0.0012906981.41078391815.659701497.053919591
17134AV10098 FWDAV10104 FWD8000AV TEL L A&Bgood 01Apr11.5DNAx 29ul0.00143751.26670992914.567164186.333549643
1851AV10104 RVSAV10106 RVS9618AV_TEL_L_Bgood 16Mar11DNAx 29ul0.0011436891.5921248317.513373137.960624152
19111AV10106 FWDAV10108.2 RVS13400AV_TEL_L_Bgood 29Mar6.1DNAx 29ul0.000455224424.420
20112AV10107.1 FWDAV10108 RVS7900AV TEL L Bgood 29Mar6.4DNAx 29ul0.0008101272.2476679114.3850746311.23833955
2155AV10108 FWDAV10111 RVS14846AV_TEL_L_Bgood 16Mar5.4DNAx 29ul0.0003637345.00611387527.0330149325.03056938
22117AV10111 FWDAV10113 RVS8400AV TEL L Bgood 29Mar10.2DNAx 29ul0.0012142861.49956101815.295522397.497805092
23128AV10113 FWDTEL_L_B_3 RVS11500AV TEL L Bgood 01Apr15.3DNAx 29ul0.0013304351.36864696120.940298516.843234806
24131AV10114 FWDTEL_L_B_4 RVS8500AV TEL L Bgood 01Apr15.3DNAx 29ul0.00181.01160862415.477611945.058043118
25138AV10083 FWDAV10084.2 RVS5400AV TEL L Agood 05Apr1010.3DNAx 29ul0.0019074070.9546442549.8328358214.773221272
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