Nuclear Region Adenita Culture 454 Amplification
Adenita Cultures 41-44
EX Takara RXN Mixture
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conc
|
1X
|
5
|
'
|
'
|
Milli Q water |
|
5.15 |
25.75 |
|
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Takara EX Buffer |
10X |
1 |
5 |
|
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dNTP |
2.5uM |
0.8 |
4 |
|
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Primer Mix |
20uM |
1 |
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EX Taq |
5U |
0.05 |
0.25 |
|
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Template |
Instagene |
2 |
10 |
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45 |
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9.0ul / RXN add 1.0ul Primer |
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PCR cycle |
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98C |
10 sec |
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60C |
1 min |
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x35 |
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AMP Lane |
Template |
Primer |
AMP Lane |
Template |
Primer
|
1 |
41 Instagene |
B5 |
16 |
44 Instagene |
B8
|
2 |
41 Instagene |
C5 |
17 |
44 Instagene |
C8
|
3 |
41 Instagene |
D5 |
18 |
44 Instagene |
D8
|
4 |
41 Instagene |
E5 |
19 |
44 Instagene |
E8
|
5 |
41 Instagene |
F5 |
20 |
44 Instagene |
F8
|
6 |
42 Instagene |
B6 |
21 |
Neg Control |
B5
|
7 |
42 Instagene |
C6 |
22 |
Neg Control |
C5
|
8 |
42 Instagene |
D6 |
23 |
Neg Control |
D5
|
9 |
42 Instagene |
E6 |
24 |
Neg Control |
E5
|
10 |
42 Instagene |
F6 |
25 |
Neg Control |
F5
|
11 |
43 Instagene |
B7 |
26 |
Pos Control |
B5
|
12 |
43 Instagene |
C7 |
27 |
Pos Control |
C5
|
13 |
43 Instagene |
D7 |
28 |
Pos Control |
D5
|
14 |
43 Instagene |
E7 |
29 |
Pos Control |
E5
|
15 |
43 Instagene |
F7 |
30 |
Pos Control |
F5
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*Positive Control = AV 20ng |
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Load 2.0ul Sample
Nuclear Region Adenita Culture 454 Amplification Status
CULTURE
|
PRIMER
|
COLUMN
|
GOOD
|
REDO?
|
REDO Result
|
Instagene 1 |
B1-F1 |
1 |
Y 10May |
|
|
Instagene 2 |
B2-F2 |
1 |
Y 10May |
|
|
Instagene 3 |
B3-F3 |
1 |
Y 11May |
|
|
Instagene 4 |
B4-F4 |
1 |
Y 11May |
|
|
Instagene 5 |
B5-F5 |
1 |
Y 11May |
|
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Instagene 6 |
B6-F6 |
1 |
Y 11May |
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Instagene 7 |
B7-F7 |
1 |
Y 12May |
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Instagene 8 |
B8-F8 |
1 |
Y 12May |
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Instagene 9 |
B9-F9 |
1 |
Y 12May |
|
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Instagene 10 |
B10-F10 |
1 |
|
B10, C10, F10 weak 12May |
|
Instagene 11 |
B11-F11 |
1 |
|
X |
Failed
|
Instagene 12 |
B12-F12 |
1 |
Y 12 May |
|
|
Instagene 13 |
B1-F1 |
2 |
WEAK 12May |
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Instagene 14 |
B2-F2 |
2 |
Y 12 May |
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Instagene 15 |
B3-F3 |
2 |
Y 12 May |
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Instagene 16 |
B4-F4 |
2 |
Y 12 May |
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Instagene 17 |
B5-F5 |
2 |
|
D5, E5, F5 weak 12May |
B5, C5, D5, E5, F5 13May
|
Instagene 18 |
B6-F6 |
2 |
|
X |
Failed
|
Instagene 19 |
B7-F7 |
2 |
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D7, E7, F7 weak 12May |
D7 13May
|
Instagene 20 |
B8-F8 |
2 |
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C8 |
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Instagene 21 |
B9-F9 |
2 |
|
X |
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Instagene 22 |
B10-F10 |
2 |
y 14May |
D10, E10 12May |
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Instagene 23 |
B11-F11 |
2 |
y 14May |
C11, D11, E11, F11 12May |
|
Instagene 24 |
B12-F12 |
2 |
y 14May |
X |
|
Instagene 25 |
B1-F1 |
3 |
y 14May |
D1 13May |
|
Instagene 26 |
B2-F2 |
3 |
Y 13May |
|
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Instagene 27 |
B3-F3 |
3 |
|
D6 weak 13May |
failed 14May
|
Instagene 28 |
B4-F4 |
3 |
|
B7, C7 13May |
only C4 14May
|
Instagene 29 |
B5-F5 |
3 |
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C5 13 May |
C5 14May
|
Instagene 30 |
B6-F6 |
3 |
Y 14May |
B6, C6, D6 13 May |
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Instagene 31 |
B7-F7 |
3 |
|
X |
B7, C7, E7 14May
|
Instagene 32 |
B8-F8 |
3 |
|
X |
failed
|
Instagene 33 |
B9-F9 |
3 |
|
X |
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Instagene 34 |
B10-F10 |
3 |
|
X |
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Instagene 35 |
B11-F11 |
3 |
|
X |
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Instagene 36 |
B12-F12 |
3 |
|
X |
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Instagene 37 |
B1-F1 |
4 |
|
x |
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Instagene 38 |
B2-F2 |
4 |
|
x |
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Instagene 39 |
B3-F3 |
4 |
|
x |
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Instagene 40 |
B4-F4 |
4 |
|
x |
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Instagene 41 |
B5-F5 |
4 |
|
x |
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Instagene 42 |
B6-F6 |
4 |
|
x |
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Instagene 43 |
B7-F7 |
4 |
|
x |
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Instagene 44 |
B8-F8 |
4 |
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x |
D8, E8 15May
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Instagene 45 |
B9-F9 |
4 |
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Instagene 46 |
B10-F10 |
4 |
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Instagene 47 |
B11-F11 |
4 |
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Instagene 48 |
B12-F12 |
4 |
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Instagene 49 |
B1-F1 |
5 |
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Instagene 50 |
B2-F2 |
5 |
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Instagene 51 |
B3-F3 |
5 |
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Instagene 52 |
B4-F4 |
5 |
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Instagene 53 |
B5-F5 |
5 |
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Instagene 54 |
B6-F6 |
5 |
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Instagene 55 |
B7-F7 |
5 |
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Instagene 56 |
B8-F8 |
5 |
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Instagene 57 |
B9-F9 |
5 |
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Instagene 58 |
B10-F10 |
5 |
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Instagene 59 |
B11-F11 |
5 |
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Instagene 60 |
B12-F12 |
5 |
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Instagene 61 |
B1-F1 |
6 |
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Instagene 62 |
B2-F2 |
6 |
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Instagene 63 |
B3-F3 |
6 |
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Instagene 64 |
B4-F4 |
6 |
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Instagene 65 |
B5-F5 |
6 |
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Instagene 66 |
B6-F6 |
6 |
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Instagene 67 |
B7-F7 |
6 |
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Instagene 68 |
B8-F8 |
6 |
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Instagene 69 |
B9-F9 |
6 |
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Instagene 70 |
B10-F10 |
6 |
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Instagene 71 |
B11-F11 |
6 |
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Instagene 72 |
B12-F12 |
6 |
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Instagene 73 |
B1-F1 |
7 |
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Instagene 74 |
B2-F2 |
7 |
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Instagene 75 |
B3-F3 |
7 |
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Instagene 76 |
B4-F4 |
7 |
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Instagene 77 |
B5-F5 |
7 |
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Instagene 78 |
B6-F6 |
7 |
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Instagene 79 |
B7-F7 |
7 |
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Instagene 80 |
B8-F8 |
7 |
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Instagene 81 |
B9-F9 |
7 |
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Instagene 82 |
B10-F10 |
7 |
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Instagene 83 |
B11-F11 |
7 |
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Instagene 84 |
B12-F12 |
7 |
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Instagene 85 |
B1-F1 |
8 |
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Instagene 86 |
B2-F2 |
8 |
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