User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/14

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Contents

Nuclear Region Adenita Culture 454 Amplification

Adenita Cultures 37-40

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
137 InstageneB11640 InstageneB4
237 InstageneC11740 InstageneC4
337 InstageneD11840 InstageneD4
437 InstageneE11940 InstageneE4
537 InstageneF12040 InstageneF4
638 InstageneB221Neg ControlB1
738 InstageneC222Neg ControlC1
838 InstageneD223Neg ControlD1
938 InstageneE224Neg ControlE1
1038 InstageneF225Neg ControlF1
1139 InstageneB326Pos ControlB1
1239 InstageneC327Pos ControlC1
1339 InstageneD328Pos ControlD1
1439 InstageneE329Pos ControlE1
1539 InstageneF330Pos ControlF1
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:14May10A.jpg

Nuclear Region Adenita Culture 454 Amplification

Re-Amp 2.0ul Adenita Cultures 22-25

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water5.1525.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene210
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
122 InstageneB101625 InstageneB1
222 InstageneC101725 InstageneC1
322 InstageneD101825 InstageneD1
422 InstageneE101925 InstageneE1
522 InstageneF102025 InstageneF1
623 InstageneB1121Neg ControlB1
723 InstageneC1122Neg ControlC1
823 InstageneD1123Neg ControlD1
923 InstageneE1124Neg ControlE1
1023 InstageneF1125Neg ControlF1
1124 InstageneB1226Pos ControlB1
1224 InstageneC1227Pos ControlC1
1324 InstageneD1228Pos ControlD1
1424 InstageneE1229Pos ControlE1
1524 InstageneF1230Pos ControlF1
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:14May10B.jpg

Nuclear Region Adenita Culture 454 Amplification

Re-Amp 2.0ul Adenita Cultures 27-32

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water5.1525.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene210
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
127 InstageneB32131 InstageneB7
227 InstageneC32231 InstageneC7
327 InstageneD32331 InstageneD7
427 InstageneE32431 InstageneE7
527 InstageneF32531 InstageneF7
628 InstageneB42632 InstageneB8
728 InstageneC42732 InstageneC8
828 InstageneD42832 InstageneD8
928 InstageneE42932 InstageneE8
1028 InstageneF43032 InstageneF8
1129 InstageneB531Neg ControlB3
1229 InstageneC532Neg ControlC3
1329 InstageneD533Neg ControlD3
1429 InstageneE534Neg ControlE3
1529 InstageneF535Neg ControlF3
1630 InstageneB636Pos ControlB3
1730 InstageneC637Pos ControlC3
1830 InstageneD638Pos ControlD3
1930 InstageneE639Pos ControlE3
2030 InstageneF640Pos ControlF3
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:14May10C.jpg

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