User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/14

Alien genes

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Nuclear Region Adenita Culture 454 Amplification Adenita Cultures 37-40 EX Takara RXN Mixture conc 1X 5 ' ' Milli Q water 4.15 20.75 Takara EX Buffer 10X 1 5 dNTP 2.5uM 0.8 4 Primer Mix 20uM 1 EX Taq 5U 0.05 0.25 Template Instagene 3 15 45 9.0ul / RXN add 1.0ul Primer PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer AMP Lane Template Primer 1 37 Instagene B1 16 40 Instagene B4 2 37 Instagene C1 17 40 Instagene C4 3 37 Instagene D1 18 40 Instagene D4 4 37 Instagene E1 19 40 Instagene E4 5 37 Instagene F1 20 40 Instagene F4 6 38 Instagene B2 21 Neg Control B1 7 38 Instagene C2 22 Neg Control C1 8 38 Instagene D2 23 Neg Control D1 9 38 Instagene E2 24 Neg Control E1 10 38 Instagene F2 25 Neg Control F1 11 39 Instagene B3 26 Pos Control B1 12 39 Instagene C3 27 Pos Control C1 13 39 Instagene D3 28 Pos Control D1 14 39 Instagene E3 29 Pos Control E1 15 39 Instagene F3 30 Pos Control F1 *Positive Control = AV 20ng Load 2.0ul Sample Nuclear Region Adenita Culture 454 Amplification Re-Amp 2.0ul Adenita Cultures 22-25 EX Takara RXN Mixture conc 1X 5 ' ' Milli Q water 5.15 25.75 Takara EX Buffer 10X 1 5 dNTP 2.5uM 0.8 4 Primer Mix 20uM 1 EX Taq 5U 0.05 0.25 Template Instagene 2 10 45 9.0ul / RXN add 1.0ul Primer PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer AMP Lane Template Primer 1 22 Instagene B10 16 25 Instagene B1 2 22 Instagene C10 17 25 Instagene C1 3 22 Instagene D10 18 25 Instagene D1 4 22 Instagene E10 19 25 Instagene E1 5 22 Instagene F10 20 25 Instagene F1 6 23 Instagene B11 21 Neg Control B1 7 23 Instagene C11 22 Neg Control C1 8 23 Instagene D11 23 Neg Control D1 9 23 Instagene E11 24 Neg Control E1 10 23 Instagene F11 25 Neg Control F1 11 24 Instagene B12 26 Pos Control B1 12 24 Instagene C12 27 Pos Control C1 13 24 Instagene D12 28 Pos Control D1 14 24 Instagene E12 29 Pos Control E1 15 24 Instagene F12 30 Pos Control F1 *Positive Control = AV 20ng Load 2.0ul Sample Nuclear Region Adenita Culture 454 Amplification Re-Amp 2.0ul Adenita Cultures 27-32 EX Takara RXN Mixture conc 1X 5 ' ' Milli Q water 5.15 25.75 Takara EX Buffer 10X 1 5 dNTP 2.5uM 0.8 4 Primer Mix 20uM 1 EX Taq 5U 0.05 0.25 Template Instagene 2 10 45 9.0ul / RXN add 1.0ul Primer PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer AMP Lane Template Primer 1 27 Instagene B3 21 31 Instagene B7 2 27 Instagene C3 22 31 Instagene C7 3 27 Instagene D3 23 31 Instagene D7 4 27 Instagene E3 24 31 Instagene E7 5 27 Instagene F3 25 31 Instagene F7 6 28 Instagene B4 26 32 Instagene B8 7 28 Instagene C4 27 32 Instagene C8 8 28 Instagene D4 28 32 Instagene D8 9 28 Instagene E4 29 32 Instagene E8 10 28 Instagene F4 30 32 Instagene F8 11 29 Instagene B5 31 Neg Control B3 12 29 Instagene C5 32 Neg Control C3 13 29 Instagene D5 33 Neg Control D3 14 29 Instagene E5 34 Neg Control E3 15 29 Instagene F5 35 Neg Control F3 16 30 Instagene B6 36 Pos Control B3 17 30 Instagene C6 37 Pos Control C3 18 30 Instagene D6 38 Pos Control D3 19 30 Instagene E6 39 Pos Control E3 20 30 Instagene F6 40 Pos Control F3 *Positive Control = AV 20ng Load 2.0ul Sample