User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/13

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Contents

Nuclear Region Adenita Culture 454 Amplification

Adenita Cultures 25-30

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
125 InstageneB12129 InstageneB5
225 InstageneC12229 InstageneC5
325 InstageneD12329 InstageneD5
425 InstageneE12429 InstageneE5
525 InstageneF12529 InstageneF5
626 InstageneB22630 InstageneB6
726 InstageneC22730 InstageneC6
826 InstageneD22830 InstageneD6
926 InstageneE22930 InstageneE6
1026 InstageneF23030 InstageneF6
1127 InstageneB331Neg ControlB1
1227 InstageneC332Neg ControlC1
1327 InstageneD333Neg ControlD1
1427 InstageneE334Neg ControlE1
1527 InstageneF335Neg ControlF1
1628 InstageneB436Pos ControlB1
1728 InstageneC437Pos ControlC1
1828 InstageneD438Pos ControlD1
1928 InstageneE439Pos ControlE1
2028 InstageneF440Pos ControlF1
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:13May10.jpg

Nuclear Region Adenita Culture 454 Amplification

Adenita Cultures 31-36

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
131 InstageneB72135 InstageneB11
231 InstageneC72235 InstageneC11
331 InstageneD72335 InstageneD11
431 InstageneE72435 InstageneE11
531 InstageneF72535 InstageneF11
632 InstageneB82636 InstageneB12
732 InstageneC82736 InstageneC12
832 InstageneD82836 InstageneD12
932 InstageneE82936 InstageneE12
1032 InstageneF83036 InstageneF12
1133 InstageneB931Neg ControlB7
1233 InstageneC932Neg ControlC7
1333 InstageneD933Neg ControlD7
1433 InstageneE934Neg ControlE7
1533 InstageneF935Neg ControlF7
1634 InstageneB1036Pos ControlB7
1734 InstageneC1037Pos ControlC7
1834 InstageneD1038Pos ControlD7
1934 InstageneE1039Pos ControlE7
2034 InstageneF1040Pos ControlF7
*Positive Control = AV 20ng


Load 2.0ul Sample

Image:13May10B.jpg

Nuclear Region Adenita Culture 454 Amplification

Re-Amp Adenita Cultures 11, 17, 18 ,19 with 2.0ul Instagene Template

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water5.1525.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene210
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
111 InstageneB111619 InstageneB7
211 InstageneC111719 InstageneC7
311 InstageneD111819 InstageneD7
411 InstageneE111919 InstageneE7
511 InstageneF112019 InstageneF7
617 InstageneB521Neg ControlB11
717 InstageneC522Neg ControlC11
817 InstageneD523Neg ControlD11
917 InstageneE524Neg ControlE11
1017 InstageneF525Neg ControlF11
1118 InstageneB626Pos ControlB11
1218 InstageneC627Pos ControlC11
1318 InstageneD628Pos ControlD11
1418 InstageneE629Pos ControlE11
1518 InstageneF630Pos ControlF11
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:13May10C.jpg

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