User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/12

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Contents

Nuclear Gene 454 Adenita Amplification

Re-Amp Cultures 7-10

EX Takara RXN Mixture conc 1X 5
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimer
17 InstageneB7
27 InstageneC7
37 InstageneD7
47 InstageneE7
57 InstageneF7
68 InstageneB8
78 InstageneC8
88 InstageneD8
98 InstageneE8
108 InstageneF8
119 InstageneB9
129 InstageneC9
139 InstageneD9
149 InstageneE9
159 InstageneF9
1610 InstageneB10
1710 InstageneC10
1810 InstageneD10
1910 InstageneE10
2010 InstageneF10
21Neg ControlB7
22Neg ControlC7
23Neg ControlD7
24Neg ControlE7
25Neg ControlF7
26Pos ControlB7
27Pos ControlC7
28Pos ControlD7
29Pos ControlE7
30Pos ControlF7
*Positive Control = AV 20ng


Load 2.0ul Sample

Image:12May10.jpg

Nuclear Gene 454 Adenita Amplification

Re-Amp Cultures 11-12

Amp Cultures 13-16

EX Takara RXN Mixture conc 1X 5
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimer
111 InstageneB11
211 InstageneC11
311 InstageneD11
411 InstageneE11
511 InstageneF11
612 InstageneB12
712 InstageneC12
812 InstageneD12
912 InstageneE12
1012 InstageneF12
1113 InstageneB1
1213 InstageneC1
1313 InstageneD1
1413 InstageneE1
1513 InstageneF1
1614 InstageneB2
1714 InstageneC2
1814 InstageneD2
1914 InstageneE2
2014 InstageneF2
2115 InstageneB3
2215 InstageneC3
2315 InstageneD3
2415 InstageneE3
2515 InstageneF3
2616 InstageneB4
2716 InstageneC4
2816 InstageneD4
2916 InstageneE4
3016 InstageneF4
31Neg ControlB1
32Neg ControlC1
33Neg ControlD1
34Neg ControlE1
35Neg ControlF1
36Pos ControlB1
37Pos ControlC1
38Pos ControlD1
39Pos ControlE1
40Pos ControlF1
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:12May10B.jpg

Nuclear Gene 454 Adenita Amplification

Amp Cultures 17-24

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
117 InstageneB52622 InstageneB10
217 InstageneC52722 InstageneC10
317 InstageneD52822 InstageneD10
417 InstageneE52922 InstageneE10
517 InstageneF53022 InstageneF10
618 InstageneB63123 InstageneB11
718 InstageneC63223 InstageneC11
818 InstageneD63323 InstageneD11
918 InstageneE63423 InstageneE11
1018 InstageneF63523 InstageneF11
1119 InstageneB73624 InstageneB12
1219 InstageneC73724 InstageneC12
1319 InstageneD73824 InstageneD12
1419 InstageneE73924 InstageneE12
1519 InstageneF74024 InstageneF12
1620 InstageneB841Neg ControlB5
1720 InstageneC842Neg ControlC5
1820 InstageneD843Neg ControlD5
1920 InstageneE844Neg ControlE5
2020 InstageneF845Neg ControlF5
2121 InstageneB946Pos ControlB5
2221 InstageneC947Pos ControlC5
2321 InstageneD948Pos ControlD5
2421 InstageneE949Pos ControlE5
2521 InstageneF950Pos ControlF5
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:12May10C.jpg

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