User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/11

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Contents

Nuclear Region 454 Adenita Amplification

Adenita Cultures 3 - 12

EX Takara RXN Mixture conc 1X 60 ' '
Milli Q water4.15249
Takara EX Buffer10X160
dNTP2.5uM0.848
Primer Mix20uM1
EX Taq5U0.053
TemplateInstagene3
360
30ul mix + 15ul Template
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimerAMP LaneTemplatePrimer
13 InstageneB3319 InstageneB9
23 InstageneC3329 InstageneC9
33 InstageneD3339 InstageneD9
43 InstageneE3349 InstageneE9
53 InstageneF3359 InstageneF9
64 InstageneB43610 InstageneB10
74 InstageneC43710 InstageneC10
84 InstageneD43810 InstageneD10
94 InstageneE43910 InstageneE10
104 InstageneF44010 InstageneF10
115 InstageneB54111 InstageneB11
125 InstageneC54211 InstageneC11
135 InstageneD54311 InstageneD11
145 InstageneE54411 InstageneE11
155 InstageneF54511 InstageneF11
166 InstageneB64612 InstageneB12
176 InstageneC64712 InstageneC12
186 InstageneD64812 InstageneD12
196 InstageneE64912 InstageneE12
206 InstageneF65012 InstageneF12
217 InstageneB751Neg ControlB1
227 InstageneC752Neg ControlC1
237 InstageneD753Neg ControlD1
247 InstageneE754Neg ControlE1
257 InstageneF755Neg ControlF1
268 InstageneB856Pos ControlB1
278 InstageneC857Pos ControlC1
288 InstageneD858Pos ControlD1
298 InstageneE859Pos ControlE1
308 InstageneF860Pos ControlF1
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:11May10.jpg


Nuclear Region 454 Adenita Amplification

Re-AMP Adenita Culture 3 and 4

EX Takara RXN Mixture conc 1X 5.5
Milli Q water4.1522.825
Takara EX Buffer10X15.5
dNTP2.5uM0.84.4
Primer Mix20uM1
EX Taq5U0.050.275
TemplateInstagene316.5
49.5
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimer
13 InstageneB3
23 InstageneC3
33 InstageneD3
43 InstageneE3
53 InstageneF3
64 InstageneB4
74 InstageneC4
84 InstageneD4
94 InstageneE4
104 InstageneF4
11Neg ControlB3
12Neg ControlC3
13Neg ControlD3
14Neg ControlE3
15Neg ControlF3
16Pos ControlB3
17Pos ControlC3
18Pos ControlD3
19Pos ControlE3
20Pos ControlF3
*Positive Control = AV 20ng


Load 2.0ul Sample

Image:11May10B.jpg

Nuclear Region 454 Adenita Amplification

Re-Amp Adenita Cultures 5 & 6


EX Takara RXN Mixture conc 1X 5
Milli Q water4.1520.75
Takara EX Buffer10X15
dNTP2.5uM0.84
Primer Mix20uM1
EX Taq5U0.050.25
TemplateInstagene315
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C10 sec
60C1 min
x35
AMP LaneTemplatePrimer
15 InstageneB5
25 InstageneC5
35 InstageneD5
45 InstageneE5
55 InstageneF5
66 InstageneB6
76 InstageneC6
86 InstageneD6
96 InstageneE6
106 InstageneF6
11Neg ControlB5
12Neg ControlC5
13Neg ControlD5
14Neg ControlE5
15Neg ControlF5
16Pos ControlB5
17Pos ControlC5
18Pos ControlD5
19Pos ControlE5
20Pos ControlF5
*Positive Control = AV 20ng

Load 2.0ul Sample

Image:11May10C.jpg

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