User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/29

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Contents

Nuclear Region 454 Amplification

EX Takara RXN Mixture conc 1X 6.5 '
Milli Q water5.1533.475
Takara EX Buffer10X16.5
dNTP2.5uM0.85.2
Primer Mix20uM1
EX Taq5U0.050.325
TemplateInstagene213
58.59.0ul / RXN
A PCR cycle
98C10 sec
60C1 min
x35
Plate 3
Ithaca 2A125
Ithaca 2A226
Ithaca 2A327
Ithaca 2A428
Ithaca 2A529
Pennsylvania (PA)B430
Tsukuba 1A431
Tsukuba 1A632
Tsukuba 1B233
Tsukuba 1B434
Tsukuba 1C135
Tsukuba 1C636
Plate 4
Tsukuba 3D237
Tsukuba 3D338
Tsukuba 3D439
Woods Hole 1A640
Woods Hole 1C241
Woods Hole 1C342
YokohamaB543
BaltimoreA344
BaltimoreA645
E. WindsorD446
HarvardA147
Ithaca 2A648
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