User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/24

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Alien Genes New Primer Screen

Reaction mixture conc. 1x x6.5 x8.5 x2 ' ' '
Temp (39 - 41)??21317
TopTaq Buffer10x16.58.5
dNTP mix10 mM each0.21.31.7
Primer mix5 uM each1
TopTaq5 U/ul0.050.3250.425
Milli Q5.7537.37548.875
10 ul
No.Primer mixNo.Primer mix
PCR cycle (2 STEP)TaKaRa Dice1AV100451AV10134
94C3 min2AV100442AV10121
3AV100433AV10084
98C10 sec4AV100424AV10045
60C1 min5AV100415AV10044
x356AV100346AV10034
7AV10027
72C5 minNo.Sample8AV10025
39Tsukuba 3D4
No.Sample
40Woods Hole 1A6
41Woods Hole 1C2

Loaded 2.0 ul of each sample

Image:0041.jpg

Loaded 8.0 ul of each sample

Image:0043.jpg


Reaction mixture conc. 1x x97 ' No. Primer mix
Temp (49 - 60)??21941AV10134
TopTaq Buffer10x1972AV10121
dNTP mix10 mM each0.219.43AV10084
Primer mix5 uM each14AV10045
TopTaq5 U/ul0.054.855AV10044
Milli Q5.75557.756AV10034
10 ul7AV10027
8AV10025
PCR cycle
94C3 minNo.
1Pennsylvania (PA) D4
98C10 sec2Tsukuba 1 B5
60C3 min3Yokohama A2
x354Baltimore C1
5Ithaca 1 A4
72C5 min6Pennsylvania (PA) B6
7Yokohama B2
8Yokohama A4
9Ithaca 1 C4
10Ithaca 1 D5
11Baltimore C5
12E. Windsor A2

Loaded 2.0ul of each sample

Image:0045-1.jpg

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