Alien Genes New Primer Screen
Reaction mixture
|
conc.
|
1x
|
x6.5
|
x8.5 x2
|
'
|
'
|
'
|
Temp (39 - 41) |
?? |
2 |
13 |
17 |
|
|
|
TopTaq Buffer |
10x |
1 |
6.5 |
8.5 |
|
|
|
dNTP mix |
10 mM each |
0.2 |
1.3 |
1.7 |
|
|
|
Primer mix |
5 uM each |
1 |
|
|
|
|
|
TopTaq |
5 U/ul |
0.05 |
0.325 |
0.425 |
|
|
|
Milli Q |
|
5.75 |
37.375 |
48.875 |
|
|
|
|
|
10 ul |
|
|
|
|
|
|
|
|
No. |
Primer mix |
|
No. |
Primer mix
|
PCR cycle (2 STEP) |
TaKaRa Dice |
|
1 |
AV10045 |
|
1 |
AV10134
|
94C |
3 min |
|
2 |
AV10044 |
|
2 |
AV10121
|
|
|
|
3 |
AV10043 |
|
3 |
AV10084
|
98C |
10 sec |
|
4 |
AV10042 |
|
4 |
AV10045
|
60C |
1 min |
|
5 |
AV10041 |
|
5 |
AV10044
|
x35 |
|
|
6 |
AV10034 |
|
6 |
AV10034
|
|
|
|
|
|
|
7 |
AV10027
|
72C |
5 min |
|
No. |
Sample |
|
8 |
AV10025
|
|
|
|
39 |
Tsukuba 3D4 |
|
|
|
|
|
|
|
|
|
No. |
Sample
|
|
|
|
|
|
|
40 |
Woods Hole 1A6
|
|
|
|
|
|
|
41 |
Woods Hole 1C2
|
|
Loaded 2.0 ul of each sample
Loaded 8.0 ul of each sample
Reaction mixture
|
conc.
|
1x
|
x97
|
'
|
No.
|
Primer mix
|
Temp (49 - 60) |
?? |
2 |
194 |
|
1 |
AV10134
|
TopTaq Buffer |
10x |
1 |
97 |
|
2 |
AV10121
|
dNTP mix |
10 mM each |
0.2 |
19.4 |
|
3 |
AV10084
|
Primer mix |
5 uM each |
1 |
|
|
4 |
AV10045
|
TopTaq |
5 U/ul |
0.05 |
4.85 |
|
5 |
AV10044
|
Milli Q |
|
5.75 |
557.75 |
|
6 |
AV10034
|
|
|
10 ul |
|
|
7 |
AV10027
|
|
|
|
|
|
8 |
AV10025
|
PCR cycle |
|
|
|
|
|
|
94C |
3 min |
|
|
|
No. |
|
|
|
|
|
|
1 |
Pennsylvania (PA) D4
|
98C |
10 sec |
|
|
|
2 |
Tsukuba 1 B5
|
60C |
3 min |
|
|
|
3 |
Yokohama A2
|
x35 |
|
|
|
|
4 |
Baltimore C1
|
|
|
|
|
|
5 |
Ithaca 1 A4
|
72C |
5 min |
|
|
|
6 |
Pennsylvania (PA) B6
|
|
|
|
|
|
7 |
Yokohama B2
|
|
|
|
|
|
8 |
Yokohama A4
|
|
|
|
|
|
9 |
Ithaca 1 C4
|
|
|
|
|
|
10 |
Ithaca 1 D5
|
|
|
|
|
|
11 |
Baltimore C5
|
|
|
|
|
|
12 |
E. Windsor A2
|
|
Loaded 2.0ul of each sample
|