User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/16

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Alien Genes LongPCR

Amplification of AV240_B, AV_TEL_L_B

' [CONC] 1X 13 '
Water5.368.9
Takara LA Taq5U0.11.3
Takara LA Buffer10X113
dNTP2.5uM each1.620.8
*Template1ng113
Primer Mix5uM each1
10.0ul1179.0ul/RXN
add 1.0 Primer Mix
* AV or CCA3
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X


Load 1.0ul sample Image:  16mar10.tif

AMP Lane Template Contents ' Fosmid MIX
1AVAV10037 FWDAV10039 RVSAV240_B47
2AVAV10036 FWDAV10041 RVSAV240_B48
3AVAV10037 FWDAV10041 FWDAV240_B49
4AVAV10039 FWDAV10041 FWDAV240_B50
5AVAV10104 RVSAV10106 RVSAV_TEL_L_B51
6AVAV10106 FWDAV10108 RVSAV_TEL_L_B52
7AVAV10104 RVSAV10108 RVSAV_TEL_L_B53
8AVAV10106 FWDAV 10109 FWDAV_TEL_L_B54
9AVAV10108 FWDAV10111 RVSAV_TEL_L_B55
10AVAV10109 RVSAV10111 RVSAV_TEL_L_B56
11AVAV10111 FWDAV10114 RVSAV_TEL_L_B57
12AVAV10108 FWDAV10109 FWDAV_TEL_L_B58
13CCA3AV10037 FWDAV10039 RVSAV240_B47
14CCA3AV10036 FWDAV10041 RVSAV240_B48
15CCA3AV10037 FWDAV10041 FWDAV240_B49
16CCA3AV10039 FWDAV10041 FWDAV240_B50
17CCA3AV10104 RVSAV10106 RVSAV_TEL_L_B51
18CCA3AV10106 FWDAV10108 RVSAV_TEL_L_B52
19CCA3AV10104 RVSAV10108 RVSAV_TEL_L_B53
20CCA3AV10106 FWDAV 10109 FWDAV_TEL_L_B54
21CCA3AV10108 FWDAV10111 RVSAV_TEL_L_B55
22CCA3AV10109 RVSAV10111 RVSAV_TEL_L_B56
23CCA3AV10111 FWDAV10114 RVSAV_TEL_L_B57
24CCA3AV10108 FWDAV10109 FWDAV_TEL_L_B58
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