User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/15

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Alien Genes Long PCR

Amplification of AV240B, AV_TEL_L_A

' [CONC] 1X 10 '
Water5.353
Takara LA Taq5U0.11
Takara LA Buffer10X110
dNTP2.5uM each1.616
*Template1ng110
Primer Mix5uM each1
10.0ul909.0ul/RXN
add 1.0 Primer Mix
* AV or CCA3
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X


Load 1.0ul sample Image:  15march10.tif

AMP Lane Template Contents ' Fosmid MIX
1AVAV10034 FWDAV10036 RVSAV240_B38
2CCA3AV10034 FWDAV10036 RVSAV240_B38
3AVAV10034 FWDAV10037 RVSAV240_B39
4CCA3AV10034 FWDAV10037 RVSAV240_B39
5AVAV10034 FWDAV10039 RVSAV240_B40
6CCA3AV10034 FWDAV10039 RVSAV240_B40
7AVAV10036 FWDAV10040.1 RVSAV240_B41
8CCA3AV10036 FWDAV10040.1 RVSAV240_B41
9AVAV10037 FWDAV10040.1 RVSAV240_B42
10CCA3AV10037 FWDAV10040.1 RVSAV240_B42
11AVAV10039 FWDAV10040.1 RVSAV240_B43
12CCA3AV10039 FWDAV10040.1 RVSAV240_B43
13AVAV10084 FWDAV10092.2 RVSAV_TEL_L_A44
14CCA3AV10084 FWDAV10092.2 RVSAV_TEL_L_A44
15AVAV10092.2 FWDAV10100 RVSAV_TEL_L_A45
16CCA3AV10092.2 FWDAV10100 RVSAV_TEL_L_A45
17AVAV10108 FWDAV10114 RVSAV_TEL_L_B46
18CCA3AV10108 FWDAV10114 RVSAV_TEL_L_B46
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