User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/09

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Contents


Alien Gene New Primer Screen

Reaction mixture conc. 1x 12
Template CCA31ng/ul112
TopTaq buffer10x112
dNTP mix10 mM each0.22.4
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.050.6
Milli Q water7.2587
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min


Load 5.0ul sample Image:  09march10.tif

AMP Lane Contents Fosmid
1AV10081AV_TEL_L_A
2AV10086AV_TEL_L_A
3AV10091AV_TEL_L_A
4AV10092.2AV_TEL_L_A
5AV10093.1AV_TEL_L_A
6AV10093.2AV_TEL_L_A
7AV100100AV_TEL_L_A
8AV10106AV_TEL_L_B
9AV10108AV_TEL_L_B
10AV10111AV_TEL_L_B
11POS Control AV10008
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