User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/05

Alien genes

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Alien Genes New Primer Screen Lane Gene Lane Gene 1 AV10158 17 AV10084 2 AV10153 18 AV10082 3 AV10151 19 AV10075 4 AV10150 20 AV10071 5 AV10140 21 AV10045 6 AV10134 22 AV10043 7 AV10130 23 AV10042 8 AV10123 24 AV10041 9 AV10121 25 AV10034 10 AV10104 26 AV10028 11 AV10109 27 AV10027 12 AV10099 28 AV10025 13 AV10098 29 AV10016 14 AV10094 30 AV10011 15 AV10092 31 AV10007 16 AV10089 32 AV10002 Reaction mixture conc. 1x x33 Temp (Tsukuba(A4,A6,B4,B5,C1,C6)) ?? 2 66 TopTaq buffer 10x 1 33 dNTP mix 10 mM each 0.2 6.6 Primer mix 5 uM each 0.5 TopTaq (QIAGEN) 5U/ul 0.05 1.65 Milli Q 6.25 206.25 10 ul PCR cycle 94C 3 min 98C 10 sec 60C 3 min x35 72C 5 min Loaded 2.0ul of sample (forgot to load ladders) Loaded 2.0ul of sample