User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/04

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Contents

Alien Genes New Primer Screen

Lane Gene Lane Gene
1AV1015817AV10084
2AV1015318AV10082
3AV1015119AV10075
4AV1015020AV10071
5AV1014021AV10045
6AV1013422AV10043
7AV1013023AV10042
8AV1012324AV10041
9AV1012125AV10034
10AV1010426AV10028
11AV1010927AV10027
12AV1009928AV10025
13AV1009829AV10016
14AV1009430AV10011
15AV1009231AV10007
16AV1008932AV10002
Reaction mixture conc. 1x x33
Temp (Baltimore C3,E.Windsor A1,A3,Ithaca2 A4,A5,Pennsylvania(PA)B4)??266
TopTaq buffer10x133
dNTP mix10 mM each0.26.6
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.051.65
Milli Q6.25206.25
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 2.0ul of sample

Image:  4Mar'10_Baltimore(C3),E.Windsor(A1,A3).jpg

Loaded 2.0ul of sample

Image:  4Mar'10_Ithaca2(A4,A5),Pennsylvania(PA)(B4).jpg

Alien Genes LongPCR

Amplification of AV212_A, AV240_B

' [CONC] 1X 15 '
Water5.379.5
Takara LA Taq5U0.11.5
Takara LA Buffer10X115
dNTP2.5uM each1.624
*Template1ng115
Primer Mix5uM each1
10.0ul1359.0ul/RXN
add 1.0 Primer Mix
* AV or CCA3
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X
AMP Lane Template Contents ' Fosmid MIX
1AVAV10007 FWDAV10011 RVSAV212_A1
2AVAV10025 FWDAV10028 RVSAV212_A4
3AVAV10004 FWDAV10007 RVSAV212_A23
4AVAV10016 FWDAV10020 RVSAV212_A24
5AVAV10020 FWDAV10025 RVSAV212_A25
6AVAV10029 FWDAV10032 RVSAV240_B26
7AVAV10032 FWDAV10034 RVSAV240_B27
8AVAV10034 FWDAV10040-1 RVSAV240_B28
9AVAV10040-1 FWDAV10041 RVSAV240_B29
10AVEU643474 FWDEU643474 RVSAV240_B30
11AVAV10045 FWDAV10047 RVSAV240_B31
12AVAV10047 FWDAV10049 RVSAV240_B32
13AVAV10049 FWDAV10053 RVSAV240_B33
14CCA3AV10007 FWDAV10011 RVSAV212_A1
15CCA3AV10025 FWDAV10028 RVSAV212_A4
16CCA3AV10004 FWDAV10007 RVSAV212_A23
17CCA3AV10016 FWDAV10020 RVSAV212_A24
18CCA3AV10020 FWDAV10025 RVSAV212_A25
19CCA3AV10029 FWDAV10032 RVSAV240_B26
20CCA3AV10032 FWDAV10034 RVSAV240_B27
21CCA3AV10034 FWDAV10040-1 RVSAV240_B28
22CCA3AV10040-1 FWDAV10041 RVSAV240_B29
23CCA3EU643474 FWDEU643474 RVSAV240_B30
24CCA3AV10045 FWDAV10047 RVSAV240_B31
25CCA3AV10047 FWDAV10049 RVSAV240_B32
26CCA3AV10049 FWDAV10053 RVSAV240_B33

Load 2.0ul sample Image:  04March10 PCR.tif

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