User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/03

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Contents

Alien Genes New Primer Screen

Screened on CCA3

Reaction mixture conc. 1x 16
Template1ng/ul116
TopTaq buffer10x116
dNTP mix10 mM each0.23.2
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.050.8
Milli Q7.25116
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 1.0ul of sample

AMP Lane Primer
1AV10004
2AV10008
3AV10010
4AV10020
5AV10022
6AV10029
7AV10032
8AV10040.1
9AV10040.2
10AV10047
11AV10049
12AV10053
13AV10087
14AV10095
15AV10114

Alien Genes New Primer Screen

Lane Gene Lane Gene
1AV1015817AV10084
2AV1015318AV10082
3AV1015119AV10075
4AV1015020AV10071
5AV1014021AV10045
6AV1013422AV10043
7AV1013023AV10042
8AV1012324AV10041
9AV1012125AV10034
10AV1010426AV10028
11AV1010927AV10027
12AV1009928AV10025
13AV1009829AV10016
14AV1009430AV10011
15AV1009231AV10007
16AV1008932AV10002
Reaction mixture conc. 1x x33
Temp (Ithaca1 B5,C1,D1)??266
TopTaq buffer10x133
dNTP mix10 mM each0.26.6
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.051.65
Milli Q6.25206.25
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 2.0ul of sample

Image: 3Mar'10 Ithaca1(B5,C1,D1).jpg‎

Reaction mixture conc. 1x x33
Temp (Ithaca1 D3,D4,D6)??266
TopTaq buffer10x133
dNTP mix10 mM each0.26.6
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.051.65
Milli Q6.25206.25
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 2.0ul of sample

Image: 3Mar'10 Ithaca1(D3,D4,D6).jpg‎

Reaction mixture conc. 1x x33
Temp (Ithaca2 A1,A2,A3)??266
TopTaq buffer10x133
dNTP mix10 mM each0.26.6
Primer mix5 uM each0.5
TopTaq (QIAGEN)5U/ul0.051.65
Milli Q6.25206.25
10 ul
PCR cycle
94C3 min
98C10 sec
60C3 min
x35
72C5 min

Loaded 2.0ul of sample

Image: 3Mar'10 Ithaca2(A1,A2,A3).jpg‎‎

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