User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/18

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Contents

Alien Genes LongPCR

Amplification of AV212_A, AV240_B, AV_TEL_L_A

' [CONC] 1X 5X
Water5.326.5
Takara LA Taq5U0.10.5
Takara LA Buffer10X15
dNTP2.5uM each1.68
Template0.5ng15
Primer Mix5uM each1
10.0ul45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
0.2ng
*AV0.5ng
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X
72C10 min

Loaded 2.0ul of sample

Lane Template Contents ' Fosmid MIX
1CCA3AV10016 FWDAV10027RVSAV212_A5
2AVAV10016 FWDAV10027RVSAV212_A5
3CCA3AV10041 FWDAV10045 RVSAV240_B7
4AVAV10041 FWDAV10045 RVSAV240_B7
5CCA3AV10082 FWDAV10084 RVSAV_TEL_L_A8
6AVAV10082 FWDAV10084 RVSAV_TEL_L_A8
7CCA3AV10092 FWDAV10099 RVSAV_TEL_L_A9
8AVAV10092 FWDAV10099 RVSAV_TEL_L_A9
9CCA3AV10099 FWDAV10109 RVSAV_TEL_L_A&B10
10AVAV10099 FWDAV10109 RVSAV_TEL_L_A&B10
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