User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/15

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Contents

Alien Genes LongPCR

Amplification of AV212_A

Test Primers on Adineta vaga DNA

' [CONC] 1X 5X
Water26.526.5
Takara LA Taq5U0.10.5
Takara LA Buffer10X15
dNTP2.5uM each1.68
Template20ng/ul15
Primer Mix5uM each1
10.0ul45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
PCR Cycle
94C 1 min
98C10 sec
68C15 min
30X
72C10 min
Lane Contents ' ' '
1100BP ladderFosmidMix
2AV10007 FWDAV10011 RVSAV212_A1
3AV10011 FWDAV10016 RVSAV212_A2
4AV10016 FWDAV10025 RVSAV212_A3
5AV10025 FWDAV10028 RVSAV212_A4

Loaded 3ul PCR product

  • Well 2,3,4 showed some evaporation
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