User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/18

From OpenWetWare

Jump to: navigation, search
Alien genes Main project page
Previous entry      Next entry

Contents

Cycle Sequencing Adineta Primer 1-36 Alien Genes

Cycle Sequencing ' ' '
ReagentsConcentration1X100X
BDT0.550
DMSO0.110
5X Seq Buffer5X0.440
H203.6360
*Primer15uM0.4
*PEG DNA variable1
TOTAL6460
Cocktail per well = 4.6ul
*add primer and PEG separately
PCR 96C10 sec
50C5 sec
60C4 min
60 cycles
PrimersSequence FWD and RVS
NAME Primer # NAME Primer # NAME Primer #
AV101581AV1009813AV1003425
AV101532AV1009214AV1002826
AV101513AV1008915AV1002727
AV101504AV1008416AV1002528
AV101405AV1008217AV1001629
AV101346AV1007518AV1001130
AV101307AV1007119AV1000731
AV101238AV1004520AV1000232
AV101219AV10044215475F+8047R33
AV1010410AV10043224220F+7822R34
AV1010911AV1004223AV10094F2+R235
AV1009912AV1004124HISR1+F136
WELL 1 2 3 4 5 6
AAV10153_Boston_A1AV10130_Boston_A4AV10123_Boston_A4AV10104_WH1_B3AV10089_WH1_B3AV10075_CC_A3
BAV10153_WH1_C4AV10130_CC_A1AV10123_WH1_B3AV10104_WH1_B5AV10089_WH1_B6AV10045_CC_A3
CAV10150_CC_A3AV10130_CC_A3AV10123_WH1_B5AV10104_WH1_B6AV10089_WH3_C2AV10045_WH3_A5
DAV10151_CC_A3AV10130_WH1_B3AV10123_WH1_B6AV10104_WH1_C4AV10084_CC_A3AV10044_CC_A3
EAV10140_CC_A3AV10130_WH1_B5AV10123_WH3_C2AV10109_CC_A3AV10084_WH1_B6AV10043_CC_A3
FAV10134_CC_A1AV10130_WH1_B6AV10121_CC_A3AV10098_Boston_A1AV10084_WH3_C2AV10042_CC_A3
GAV10134_WH3_C2AV10130_WH3_C2AV10104_CC_A1AV10098_Boston_A4AV10082_WH1_B5AV10042_WH1_B6
HAV10130_Boston_A1AV10123_Boston_A1AV10104_CC_A3AV10098_CC_A3AV10082_WH1_B6AV10042_WH3_A5
WELL789101112
AAV10041_Boston_A4AV10027_CC_A3AV10025_WH1_B65475_WH1_C4AV100942_WH1_C4HIS1_WH1_B5
BAV10041_CC_A3AV10027_WH2_A6AV10016_CC_A35475_WH3_A5AV100942_WH3_A5HIS1_WH1_B6
CAV10034_CC_A1AV10027_WH3_A5AV10011_CC_A35475_WH3_C2AV100942_WH3_C2HIS1_WH1_C4
DAV10034_CC_A3AV10027_WH3_C2AV10007_CC_A3AV100942_CC_A1HIS1_Boston_A1HIS1_WH2_A6
EAV10028_CC_A3AV10025_CC_A15475_CC_A3AV100942_CC_A3HIS1_Boston_A4HIS1_WH3_A5
FAV10028_WH1_B3AV10025_CC_A35475_WH1_B3AV100942_WH1_B3HIS1_CC_A1HIS1_WH3_C2
GAV10027_Boston_A4AV10025_WH1_B35475_WH1_B5AV100942_WH1_B5HIS1_CC_A3Sample
HAV10027_CC_A1AV10025_WH1_B55475_WH1_B6AV100942_WH1_B6HIS1_WH1_B3Sample
Personal tools